硅微通道内血细胞流动特性测试  

Flow Characteristics of Blood Cells in Silicon Microchannel

作　　者：王小章[1] 王朝晖[1] 蒋庄德[1] 叶鹏[1] 

机构地区：[1]西安交通大学机械制造系统工程国家重点实验室,西安710049

出　　处：《西安交通大学学报》2009年第5期128-132,共5页Journal of Xi'an Jiaotong University

基　　金：国家自然科学基金资助项目(50775177);国家自然科学基金重点资助项目(50535030)

摘　　要：在微型机电系统硅微制造技术制作的矩形硅微通道内注入血细胞溶液,用高速摄影机采集记录血细胞微流动图像,利用图像处理技术,对流动图像进行背景噪声滤除、对比度增强、平滑滤波、形态学运算等操作,并用提取的边缘进行图像分割及识别.按照目标识别的结果,对识别出的细胞形状通过曲线拟合法进行修正,计算出多个流动状态参数,如细胞大小、流动速度等.计算结果表明:微通道内细胞的流动速度仍近似地符合抛物线形分布,与宏观流动的流形相类似;在流体溶液的作用下,血细胞的流动状态不仅伸长变形成椭球形,而且还会产生翻滚和旋转的运动方式,并发生在细胞各自的移动路径中,使得细胞在流体中的姿态不断发生变化;细胞的翻滚和旋转产生的原因主要是血细胞自身形状所受的流动剪切力矩不平衡,而运动的结果使血细胞的流动行为更加复杂多变.The diluted blood cell solution was injected into microfabricated silicon microchannels, and a high speed complementary metal oxide semiconductor camera was employed to record the microflow video. The images extracted from the recorded video were processed and segmented with background noises removing, contrast gradient enhancing, intensity smoothing, and mathematical morphology operating to recognize the moving objects. For the errors and deviations in segmentation, the recognized cell shapes were corrected to more reasonable profiles by curve-fitting, and applied to calculate the flow parameters of cells including sizes, velocities etc. The calculated results indicated that the flow profile of cells gets approximately parabolic and similar to the common flow in large channels. Under the pulling and dragging of solution, cells not only are elongated to ellipsoidal shapes but also roll and rotate themselves to adjust orientation in fluid environment. These rolling and rotating movements only occur in their flow traces and change their flow state in microchannels. The reason why these phenomena take place is mainly due to the imbalance of the flow shear torques upon blood cell in the mieroehannel, which makes the flow characteristics of blood cells more complicated and variable.

关 键 词：微型机电系统 微流动 微通道 血细胞流动特性 

分 类 号：R318.01[医药卫生—生物医学工程]