c-Abl／Arg靶向RNA干扰对细胞周期的影响  

Effect of targeted RNA interference of c-Abl/Arg non-receptor kinase on cell cycle

作　　者：李大培[1,2] 李平[2] 曹诚[2] 张部昌[1] 刘萱[2] 

机构地区：[1]安徽大学生命科学学院,安徽省生态工程与生物技术重点实验室,合肥230039 [2]军事医学科学院生物工程研究所,北京100850

出　　处：《军事医学科学院院刊》2009年第2期106-108,119,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：军队医药卫生科研项目(No.06MA328)

摘　　要：目的:探讨非受体酪氨酸激酶c-Abl/Arg对细胞周期的影响。方法:用si-STRIKE-c-Abl/Arg siRNA表达载体转染乳腺癌细胞MCF-7,筛选具有潮霉素(hygromycin)抗性的c-Abl/Arg双敲低的稳定细胞系(c-Abl/Arg-DKD),用流式细胞仪检测其细胞周期。结果:筛选获得的c-Abl/Arg-DKD稳定细胞系中c-Abl/Arg的表达被有效抑制,且在S/G2期产生显著的细胞周期阻滞效应。结论:c-Abl非受体酪氨酸激酶在S/G2周期转换过程中发挥调节作用。Objective：To establish the c-Abl/Arg double knock-down stable cell line and investigate the effect of c-Abl/ Arg RNA on MCF-7 cell cycle progression. Methods：si-STRIKE-c-Abl/Arg Plasmid, which was directed against c-Abl/ Arg expression, was transfected into MCF-7 cells. The stable siRNA-mediated c-Abl/Arg knockdown cell lines selected by hygromycin were identified by Western blot. Cell cycle progression was detected by flow cytometry. Results：More than 90% of the decrease in c-Abl expression in c-Abl/Arg-DKD stable cell lines resulted in remarkable S/G2 cell cycle arrest. Conclusion ：The results demonstrated that c-Abl tyrosine kinase is involved in the regulation of S/G2 cell cycle transition.

关 键 词：非受体酪氨酸激酶 RNA干扰 稳定细胞系 细胞周期 

分 类 号：Q25[生物学—细胞生物学]