骨形态发生蛋白7在β淀粉样蛋白致大鼠海马神经元损害中的保护作用  

Neuroprotective Effects of BMP7 on Aβ Induced Neurotoxicity in Rat Hippocampus Neurons

作　　者：孙琳[1] 郭春妮[1] 汤建军[1] 赵圣杰[1] 王铭琴[1] 赵永波[1] 

机构地区：[1]上海交通大学附属第一人民医院神经内科上海交通大学医学院,上海200080

出　　处：《中国神经免疫学和神经病学杂志》2009年第3期191-194,198,共5页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的探讨骨形态发生蛋白7(bonemorphogenetic protein 7,BMP7)在β淀粉样蛋白(amyloid β,Aβ)致大鼠海马神经元损害中的保护作用。方法 SD大鼠海马神经元原代培养7～10 d,培养基中添加Aβ25-35毒性片段,10 min后添加不同浓度的BMP7。采用CCK 8法检测细胞活力,TUNNEL荧光半定量法检测细胞凋亡,MAP2免疫荧光法观察神经元形态。结果 Aβ+不同浓度BMP7组细胞活力均较Aβ处理组明显增高(P<0.05),并随BMP7浓度增加其活性升高;TUNNEL荧光半定量实验显示Aβ+不同浓度BMP7组凋亡细胞较Aβ处理组明显减少(P<0.05),并随BMF7浓度增加凋亡细胞呈减少趋势;MAP2免疫荧光法观察神经元形态显示,Aβ+BMP7组较Aβ处理组海马神经锥体神经元的形态保护良好。结论 BMP7在Aβ致大鼠海马神经元损害中具有保护作用,且其保护作用具有剂量依赖趋势。Objective To observe the effect of the bone morphogenetic protein （BMP7） on Aβ induced neurotoxicity in rat hippocampus neurons. Methods Hippocampal neurons of SD rats were treated by method of primary culture for 7-10 d. Cultured neurons were treated with Aβ25-35 and BMP7 for 24 hours. Cell viability was evaluated by CCK-8 test, apoptosis was detected by TUNEL staining, and the morphology of neurons was assessed by MAP-2 immunofluorescence staining. Results CCK-8 test demonstrated that the viability of neurons treated by 100-200 ng/mL BMP7 for 10 minutes was significantly higher than that of Aβ25-35 treating group （P〈0.05）, and the viability of neurons became higher with increasing the concentration of BMP7. It was supported that BMP7 decreased apoptosis after treating neurons by Aβ25-35 （P〈0.05）. A loss of MAP-2 positive dendrites after Aβ25-35 treatment was demonstrated by immunofluorescence staining, which was significantly improved after BMP7 treatment. Conclusions BMP7 significantly attenuated Aβ25-35 induced neurotoxicity, indicating that BMP7 has potential neuroprotective effects on Aβ related neuropathology.

关 键 词：阿尔茨海默病 骨形态发生蛋白7 Aβ25—35 神经保护 

分 类 号：R749.16[医药卫生—神经病学与精神病学]