大蒜新素对全身播散型人巨细胞病毒感染小鼠脏器病变的治疗作用  

Therapeutic Effects of Allitridin on Organ Pathological Changes of Systemically Disseminated MCMV Infected Mice

作　　者：葛海霞[1] 刘兴楼[1] 舒赛男[1] 李亚男[1] 罗丹[1] 方峰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《医药导报》2009年第5期555-559,共5页Herald of Medicine

基　　金：国家自然科学基金资助项目(基金编号:30572345)

摘　　要：目的观察大蒜新素对巨细胞病毒全身播散感染小鼠肝脏、肺、肾脏、心肌组织病理变化的影响,探讨大蒜新素对巨细胞病毒全身播散感染小鼠的治疗效果。方法将60只BALB/c小鼠腹腔接种MCMV(Smith株)后随机分为实验组和对照组各30只,接种MCMV,24h后实验组给予大蒜新素25mg·kg-1.d-1腹腔注射,qd;对照组给予等量0.9%氯化钠注射液,共120d。给药后1,3,7,14,28,45,60,75,90和120d两组各处死3只小鼠,显微镜下观察肝、肺、肾、心肌组织病理变化并进行评分。结果两组肝、肺和心肌组织病理评分治疗后7d达最高值,肾组织病理评分治疗后14d达最高值,以后逐渐下降。与对照组比较,实验组脏器组织病变轻。两组肝脏HAI评分治疗14d后差异有极显著性(P<0.01),以后差别逐渐增大;实验组肺组织Gloor评分给药28d后与对照组比较差异有极显著性(P<0.01);肾组织与心肌组织病理评分治疗14d后与对照组比较差异有显著性(均P<0.05),90d差异更显著(P<0.01)。此外,在给药后120d,实验组肝、肺组织接近正常,肾脏和心肌仍有一定程度病理损伤。结论大蒜新素治疗2周后能够明显减轻模型小鼠肝、肺、肾脏和心肌病理损伤,对肝脏和肺的治疗效果尤其显著。Objective To investigate the therapeutic effects of allitridin on organs pathological changes of systemically disseminated MCMV infected mice. Methods After inoculation with Smith strain of MCMV, sixty BALB/c mice were randomly divided into allitridin-treated and placebo-treated groups. The therapy started at 24 hours after the inoculation. Allitridin was given to mice via intraperitoneal （ i. p. ） route once a day at 25 mg.kg^-1 . d^-1 ; while the same volume of 0.9% sodium chloride solution was given in placebo-treated group, and both treatments lasted for 120 d. 3 mice from each group were sacrificed at 1, 3, 7, 14, 28, 45, 60, 75, 90 and 120 d respectively after therapy. The liver, lung, kidney, and myocardium were fixed, sliced at a thickness of 5 μm and stained with hematoxylin and eosin （HE） for histology examination. Results The pathological score of liver, lung and myocardium from allitridin-treated group reached peak level on day 7, and kidney on day 14, then all descended gradually. Compared with placebo-treated group, the pathological injury was ameliorated in allitridintreated group： the histological activity index （HAI） of livers from both groups were obviously different on day 14 （P 〈 0.01 ） ;the Gloor score of lungs were significantly different since day 28 （ P 〈 0.01 ） ; the difference of the pathological score of kidneys and myocardiums was significant since day 14 （P 〈 0.05 ）, and became more predominant after day 90 （P 〈 0.01 ）. Moreover, on day 120, the liver and lung of allitridin-treated mice were nearly normal, while the kidney and myocardium still had some degree of pathological injury. Conclusion After treated with allitridin for 2 weeks, the pathological injuries in liver, lung, kidney and myocardium of infected mice were ameliorated obviously, especially in liver and lung.

关 键 词：大蒜新素 巨细胞病毒 肝脏 肺 肾脏 心肌 病理评分 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]