肝细胞癌患者CD8^+T淋巴细胞上PD-1高表达的意义研究  

Significance of PD-1 expression on CD8^+ T lymphocytes from patients with hepatocellular carcinoma

作　　者：曾珍[1] 石峰 张敏娜[1] 施明 楼敏[1] 陆荫英[1] 陈艳[1] 许桂林[1] 郝莉燕[1] 王福生 杨永平[1] 

机构地区：[1]解放军第三0二医院,北京100039 [2]解放军传染病研究所,北京100039

出　　处：《传染病信息》2009年第2期83-85,共3页Infectious Disease Information

基　　金：国家重大专项基金资助项目(2008ZX10002-018);军队医学杰出人才基金资助项目(04J020)

摘　　要：目的了解细胞程序性死亡受体1(programmed death1,PD-1)在HBV相关性肝细胞癌(hepatocellular carcinoma,HCC)患者CD8+T淋巴细胞上的表达及与疾病状态的关系。方法用流式技术检测58例HBV相关性PHC患者外周血及肝组织中CD8+T淋巴细胞上PD-1的表达,并与20例HBV相关性肝硬化患者及20例健康对照者相比较。结果HCC患者外周血CD8+T淋巴细胞上PD-1的表达明显上调,与肝硬化组及健康组相比,差异有统计学意义,并且肿瘤区的表达高于非肿瘤区,随着HCC病情的进展而增加。结论PD-1在CD8+T淋巴细胞上表达具有负性调节CD8+T淋巴细胞活化增殖作用,其表达水平对HCC病情有预测作用。objective To evaluate the expression of programmed death-l（PD-1） on circulating CD8^＋ T lymphocytes from patients with HBV-related hepatocellular carcinoma（HCC） and investigate the correlation between PD-1 expression and the disease status. Methods The expression of PD-1 was measured on CD8^＋ T lymphoeytes from peripheral blood and liver tissues of 58 patients with HBV- related HCC （HCC group） by a fluorescence-activated cell sorter （FACS）, and compared with that of 20 HBV patients with liver cirrhosis （LC group） and that of 20 healthy human controls （HC group）. Results PD-1 levels on CD8＋ T lymphocytes from peripheral blood of patients in HCC group were significantly up-regulated. The differences in PD-1 levels were statistically significant among HCC group, LC group and HC group. PD-1 levels in tumor section were higer than those in non-tumor section （41.45% vs, 5.6% ）. PD-1 levels increased with the progression of the HCC stages and the disease status. Conclusions PD-1 expression up-regulated on T lymphocytes has a nega- tively regulatory effect on CD8＋ T cells. PD-1 levels can predict the disease status of HCC.

关 键 词：肝细胞癌 HBV感染 细胞程序性死亡受体1 

分 类 号：R730.3[医药卫生—肿瘤]