温热对HPV-6／11型早期基因E6和E7表达的影响  

Effects of hyperthermia on the expression of E6 and E7 genes of human papillomavirus type 6 and 11

作　　者：祝立丽[1] 高兴华[1] 李晓东[1] 马远平[1] 王雅坤[1] 王晓琴[1] 齐瑞群[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科,卫生部免疫皮肤病学重点实验室,沈阳110001

出　　处：《中华皮肤科杂志》2009年第5期305-307,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30740082）、教育部创新团队支持计划项目（IRT0760）

摘　　要：目的探讨不同温热条件对HPV感染皮损中E6／E7基因表达的影响。方法采用PCR法确定6例尖锐湿疣标本HPV型别。利用37℃，42℃，45qc不同的温热条件处理置于培养液中的离体尖锐湿疣皮损，采用实时定量PCR法检测HPV-6／11型早期基因E6／E7mRNA的表达水平。结果6例标本中，各有1例分别感染HPV-6、HPV-11，4例同时感染HPV-6和HPV-11。E6、E7 mRNA的表达水平均随温度升高而逐渐减少。HPV-6 E6 mRNA的相对表达量分别为37℃（1．00±0．00），42℃（0．61±0．17），45℃（0．27±0．15）；HPV-6 E7 mRNA的相对表达量分别为37℃（1．00±0．00），42℃（0．56±0．21），45℃（0．16±0．11）；HPV-11 E6 mRNA的相对表达量分别为37℃（1．00±0．00），42℃（0．60±0．22），45℃（0．16±0．08）；HPV-11 E7 mRNA相对表达量分别为37℃（1．00±0．00），42℃（0．55±0．15），45℃（0．24±0．06）。组间比较差异有统计学意义（P〈0．01）。结论温热对HPV-6／11型E6和E7基因表达的抑制作用可能是感染消退的机制之一。Objective To investigate the effect of hyperthermia on the expression of E6 and E7 genes of human papillomavirus （HPV） type 6 and 11 in HPV-infected human skin. Methods Tissue samples were obtained from the lesions of condyloma accuminatum （CA） in 6 patients after informed consent. Each sample was divided into 4 parts： one was embedded and directly stored at -80 ℃; the other 3 parts were placed in culture medium and the surface of the samples was irradiated for 30 minutes with a thermotherapy apparatus at 37 ℃, 42℃, 45 ℃, respectively, then the samples were taken out and stored at -80℃. RNA was extracted from the specimens, real time quantitative PCR （qPCR） was performed to detect the expression of E6 and E7 genes of HPV-6 and -11. Results Of the 6 patients, 2 were infected with HPV-6 and -11 respectively, 4 with both HPV-6 and HPV-11. The expression of E6 and E7 mRNA decreased with the increase in irradiation temperature. The relative mRNA expression levels at 37 ℃, 42℃ and 45 ℃ were 1.00 ± 0.00, 0.61 ± 0.17, 0.27 ± 0.15, respectively, for HPV-6 E6 gene, 1.00 ± 0.00, 0.56 ± 0.21, 0.16± 0.11 respectively, for HPV-6 E7 gene, 1.00 ± 0.00, 0.60 ± 0.22, 0.16±0.08, respectively, for HPV-11 E6 gene, 1.00 ±0.00, 0.55 ± 0.15, 0.24 ± 0.06, respectively, for HPV-11 E7 gene; statistical difference was noted among them between the specimens irradiated at different temperature （all P 〈 0.01 ）. Conclusion Hyperthermia can remarkably suppress the expression of HPV-6/11 E6 and E7 genes, which may be a possible mechanism under the regression of warts induced by local hyperthermia.

关 键 词：人乳头瘤病毒6 人乳头瘤病毒11 发热 乳头瘤病毒E7蛋白质类 

分 类 号：R737.33[医药卫生—肿瘤] R752.53[医药卫生—临床医学]