兔Epi—LASIK与Flap—free Epi—LASIK术后TGF—β2和b-FGF的表达及其对Haze形成的影响研究  

Research of TGF- β2 and b-FGF＇s expression after Epi-LASIK and Flap-free Epi-LASIK on rabbit and discuss the influence on Haze

作　　者：向一旻[1] 杜之渝[1] 刘玺[1] 黄正[1] 赵武校[1] 

机构地区：[1]重庆医科大学附属第二医院眼科中心,重庆400010

出　　处：《中国实用眼科杂志》2009年第5期546-548,共3页Chinese Journal of Practical Ophthalmology

摘　　要：目的观察比较Haze形成过程中的两个主要调控因子TGF-β和b—FGF，在兔Epi—LASIK与Flap—freeEpi—LASIK术后表达有否差异，并探讨其对术后Haze形成的影响。方法选用18只实验兔，建立兔Flap—freeEpi—LASIK、Epi—LASIK模型，用VISX20／20B准分子激光仪作-10D切削，在无任何药物干预的情况下观察术后1、2、3月角膜Haze情况及检测比较角膜TGF—β2和b-FGF的表达水平，并探讨其对Haze形成的影响。结果裂隙灯下观察Haze分级水平在术后1月、2月、3月差异均无统计学意义。Flap—free Epi—LASIK和Epi—LASIK术后1、2、3月TGF-β2表达差异没有统计学意义，且表现出逐渐降低的趋势。术后各时间点b-FGF表达组间比较差异无统计学意义，组内比较，b-FGF表达水平均呈现出持续升高的特点。结论Epi—LASIK与Flap—freeEpi—LASIK术后各时间点TGF-β2和b-FGF的表达情况及Haze发生程度均未见明显差异。Objective To observe and compare the main control factor TGF-β2 and b-FGF＇s expression after the operation Epi-LASIK and Flap-free Epi-LASIK on rabbit during the process of Haze forming and discuss the influence on Haze forming after the operation. Methods 18 rabbits are established as Flap-free Epi-LASIK、Epi-LASIK models.Do-10D artherectomy with VISX20/20B.Then observe the Haze of 1,2,3m cornea under the situation without any drug intervention after operation.Test and compare cornea TGF-β2 and b-FGF expression and discuss its influence on Haze forming. Results It shows that there is no statistic mean- ing of the discrepancy of Haze after 1 month, 2 months and 3 months observing with slit lamp.The expression discrepancy of 1,2,3mTGF-β2 after Flap-free Epi-LASIK ＆ Epi-LASIK is also no statistic meaning,and it presents falling trend.On each time point after operation, comparing of each b-FGF, the discrepancy is no statistic meaning.Comparing in group, the expression of b-FGF presents rising trend. Conclusion There is no obvious discrepancy of TGF-β2 and b-FGF expression and Haze after Epi-LASIK and Flap-flee Epi-LASIK.

关 键 词：EPI-LASIK Flap—free Epi—LASIK TGF-β2 b—FGF 

分 类 号：R779.6[医药卫生—眼科] R779.63[医药卫生—临床医学]