宫颈癌基质金属蛋白酶、Ⅳ型胶原和微血管密度的临床意义  

Clinical Significance of Matrix Metalloproteinase, Collagen Ⅳ and Microvessel Density in Human Cervical Carcinoma

作　　者：杨洪波[1,2] 姚海涛[1,2] 马学芝[1,2] 李丽丽[1,2] 

机构地区：[1]黑龙江省鸡西矿业集团总医院妇产科,黑龙江鸡西158100 [2]黑龙江省佳木斯大学医学部病理科,黑龙江佳木斯154000

出　　处：《现代生物医学进展》2009年第9期1668-1672,共5页Progress in Modern Biomedicine

摘　　要：目的:研究宫颈癌中MMP-2、MMP-9、Ⅳ型胶原表达和微血管密度的临床意义。方法:采用SABC免疫组织化学方法对64例子宫颈癌组织(全部为鳞癌),20例正常宫颈组织进行MMP-2、MMP-9,Ⅳ型胶原和微血管密度的检测。结果:(1)MMP-2,MMP-9二者在正常宫颈组织、宫颈癌(ICC)的细胞浆的阳性表达率分别为:MMP-2阳性20.0%(4/20)、75.0%(48/64);MMP-9的阳性为:15.0%(3/20)、78.1%(50/64),MMP-2、MMP-9的正常组与宫颈癌组相比差异有显著性(p<0.05)。(2)Ⅳ型胶原表达随着宫颈癌的进展、分期升级,基底膜破坏程度明显增加而显著减少,正常组织与宫颈癌之间差异显著。有淋巴结转移者Ⅳ型胶原表达则明显低于无淋巴结转移组(p<0.005)。(3)微血管密度与宫颈癌的恶性程度及淋巴结转移密切相关,宫颈癌患者的微血管密度明显高于对照组,在临床II、III期明显高于I期。MVD与肿瘤大小、病理分级无关。结论:MMP-2、MMP-9活性表达与Ⅳ型胶原的表达负相关,与MVD正相关。Ⅳ型胶原的表达与宫颈癌的临床分期及有无淋巴结转移相关,可作为判定宫颈癌浸润及转移程度的一个生物学指标。微血管密度与宫颈癌的恶性程度及淋巴结有无转移密切相关。因此采用MVD评价宫颈癌预后是有意义的。Objective： To investigate the clinical significance of MMP-2,MMP-9, Ⅳ collagen expression and microvessel density in cervical carcinoma. Methods： 64 cases of cervical carcinoma and 20 cases of normal cervical epithelium were examined for expression of MMP-2,MMP-9, IV collagen and microvessel density by immunohistochemistry SABC method. Results： （1）The expression positive rates of MMP-2,MMP-9 in normal cervical epithelium, squamous-cell carcinoma were 20.0% （4/20）,75.0% （48/64）; 15.0% （ 3/20）,78.1% （50/64）, respectively. The positive rates of MMP-2 and MMP-9 in invasive cervical carcinoma groups were obviously higher than in normal cervical epithelium group （p〈0.05）. （2）The expression rates of Ⅳ collagen were gradually decreased with the progress cervical lesion and the basement membrane destroy .The expression of Ⅳ collagen in invasive cervical carcinoma groups were obviously higher than in normal cervical epithelium group （p〈0.05）. The expression of Ⅳ collagen in metastasis were obviously lower than without metastasis. （p〈0.005）.（3） Microvessel density had a close correlation with degree of malignant of cervical carcinoma and lymph-node metastasis. Our results showed that density ofmicrovessel in cervical carcinoma was higher than in the control group, and that in stage Ⅱ, Ⅲ were much higher than in stage I. No significant correlation could be established among the expression of MVD and tumor diameter and pathologic grade. Conclusions： There were negatively correlations between MMP-2, MMP-9 and Ⅳ collagen, on the contrary between MMP-2,MMP-9 and MVD. The expression of Ⅳ collagen in invasive cervical carcinoma groups has a close correlation with clinical stages and lymph-node metastasis. Ⅳ collagen may be a useful mark in predicating lymphnode metastasis and prognosis. Microvessel density has a close correlation with degree of malignant of cervical carcinoma and lymph-node metastasis. It could be served as indicator for predicting prognosis.

关 键 词：宫颈癌 MMP-2 MMP-9 Ⅳ型胶原 微血管密度 免疫组化 

分 类 号：R737.33[医药卫生—肿瘤]