正交试验法优选舒心胶囊原料药中挥发油的提取工艺  

Extraction Process of Volatile Oil in the Raw Materials of Shuxin Capsule selected by Orthogonal experiment

作　　者：杨剑芳[1] 许利平[2] 王欣苗[2] 于萍[2] 王燕 穆阳[2] 庞鑫 

机构地区：[1]北京市农林科学院植物保护环境保护研究所,北京100097 [2]首都医科大学中医药学院,北京100069 [3]北京海虹药通电子商务有限公司,北京100120 [4]北京市门头沟区中医医院药剂科,北京102300

出　　处：《现代生物医学进展》2009年第9期1739-1741,共3页Progress in Modern Biomedicine

基　　金：北京市优秀人才培养专项经费D类资助(No.820039);北京市属市管高等学校人才强教计划资助项目(No.BAHED)

摘　　要：目的:探索舒心胶囊原料药中挥发油的提取工艺。方法:采用高效液相色谱法,用70%甲醇回流提取制样用作阿魏酸含量测定的方法学考察;用水蒸气蒸馏法提取挥发油,以挥发油提取率和阿魏酸含量为指标,采用L(934)正交试验设计,筛选挥发油的最佳提取工艺。结果:舒心胶囊原料药中阿魏酸含量为0.070%。影响提取的主次因素为:浸泡时间>加水量>提取时间;优选得到的最佳提取条件为:药材不必浸泡,加10倍量水(V/W)后提取3h。结论:该工艺准确、稳定,重复性好。Objective： To explore the optimal extraction process of volatile oil in the raw materials of Shuxin Capsule. Methods： The measurement method of ferulic acid was determined by HPLC using samples refluxed with 70% methanol. The volatile oil was extracted by vapor distillation method and the optimum extraction conditions were investigated by orthogonal design using the extraction rate of volatile oil and the content of ferulic acid as two indexes. Results： The content of ferulic acid in the raw materials of Shuxin Capsule was 0.070%. The factors influencing the effect in decreasing order were soaking time〉water amount 〉extraction time; thus the optimal extraction condition was as follows： the raw materials were added 10 times of water and extracted for 3 h without soaking. Conclusions： This method is accurate, stable, and repeatable.

关 键 词：舒心胶囊 挥发油 阿魏酸 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]