细菌调节蛋白Hfq研究进展  

Research on the Post-transcriptional Regulator Hfq in Bacteria

作　　者：苗翠翠[1] 程汝滨[1] 宫倩红[1] 于文功[1] 

机构地区：[1]中国海洋大学医药学院分子生物学实验室,山东青岛266003

出　　处：《现代生物医学进展》2009年第9期1778-1780,共3页Progress in Modern Biomedicine

基　　金：国家863重点项目(2007AA091506)

摘　　要：Hfq是一个高度保守的RNA结合蛋白,最初被发现是作为大肠杆菌(E.coli)RNA噬菌体Qβ复制所必需的管家因子,现在则被认为是细菌基因转录后调控的关键因子,广泛参与细菌多种生命活动的调控。与真核生物中Sm和Sm样蛋白相似,Hfq可以通过形成同源六聚体结合富含A、U的单链RNA参与RNA间互作。同时Hfq蛋白还可与体内的多种RNA调节蛋白如polyA聚合酶(IPAPI)、多聚核苷酸磷酸化酶(PNP)、RNA酶E(RNaseE)等并调节他们的活性。此外,Hfq对自身的表达也具有回馈抑制作用。本文主要结合Hfq的研究历史和最新进展,对其分子结构、作用机制、生理功能以及系统进化中的地位做一综述。The conserved RNA-binding protein Hfq, originally discovered in Escherichia coli as a host factor for Qβ replicase, has emerged as a global post-transcriptional regulator in bacteria. It shares functional and structural features with the eukaryotic Sm and Sm-like proteins, and binds to AU-rich RNAs to assist in bimolecular RNA-RNA interactions by forming ring-shaped hetroheptamers. In addition to binding RNAs, Hfq has also been shown to interact with a variety of proteins that are involved in mRNA decay, for example, poly（A） polymerase Ⅰ（PAPⅠ）, polynucleotide phosphorylase （PNP）, RNase E and so on. Additionally, Hfq modulates its expression by translating repression ofhfq mRNA. In this review, we mainly summarized the history research of Hfq and discussed recent advanees on insight into the structure, mechanism, physiology, and evolution of Hfq.

关 键 词：HFQ 转录后调控 系统进化 

分 类 号：Q75[生物学—分子生物学] R37[医药卫生—病原生物学]