Construction of non-replicating recombinant vaccinia virus expressing HPV16 L1,L2E7 proteins  

表达HPV16L1、L2和E7蛋白的非复制重组痘苗病毒的构建(英文)

作　　者：Jiangtao Fan Xinqiu Chen Wei Huang Houwen Tian 

机构地区：[1]Department of Gynecology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China [2]Department of Gynecological Tumor, The Affiliated Oncology Hospital of Guangxi Medical University, Nanning 530021, China [3]Institute of Virology, Chinese Center for Disease Control and Prevention, Beijing 100023, China

出　　处：《The Chinese-German Journal of Clinical Oncology》2009年第5期247-250,共4页中德临床肿瘤学杂志（英文版）

基　　金：Support by a grant from the National High Technology Research and Development Program, China (No.2002AA216041).

摘　　要：Objective： To construct a non-replicating vaccinia virus expressing human papillomavirus 16 （HPV16） L1, L2E7 proteins as a candidate vaccine for cervical cancer. Methods： Using vaccinia virus vector, we generated a strain of non-replicating recombinant vaccinia virus vaccine expressing HPV16 L1, L2E7 proteins by homologous recombination and identified by PCR and Westernloting. Results： We demonstrated that the L1, L2E7 gene of HPV16 were integrated into vaccinia genosome and could express L1, L2E7 protein stably when infected the CEF using PCR and Western-blot assay. Conclusion： NTVJL1/L2E7 can express L1, L2E7 protein of HPV16 and can be taken as a candidate vaccine for HPV16-associated diseases.

关 键 词：human papillomavirus vaccinia virus VACCINE 

分 类 号：R373[医药卫生—病原生物学] R392.13[医药卫生—基础医学]