急性白血病患者P-糖蛋白、抗凋亡因子和肺耐药相关蛋白表达及临床意义  

Expression of Pgp,Bcl-2 and LRP in acute leukemia and clinical significance

作　　者：肖镇[1] 黄彬涛[1] 哈森[1] 石玉涛[2] 高大[1] 

机构地区：[1]内蒙古医学院附属医院血液科,呼和浩特010059 [2]内蒙古自治区医院血液科

出　　处：《临床血液学杂志》2009年第3期241-243,共3页Journal of Clinical Hematology

摘　　要：目的:通过对急性白血病(AL)患者外周血中P-糖蛋白(P-gp)、抗凋亡因子(Bcl-2)、肺耐药相关蛋白(LRP)表达的检测,探讨蛋白表达与完全缓解率之间的关系,耐药蛋白在正常对照组、初治组中的表达是否存在差异,外周血高白细胞数与耐药蛋白高表达的相关性。方法:应用免疫组织化学SP法检测LRP、Pgp、Bcl-2的表达,采用SPSS10.0统计软件进行χ2检验、线性相关分析统计结果。结果:白血病患者耐药蛋白表达阳性较阴性者完全缓解率降低。Pgp/Bcl-2共同阳性表达者,较某一蛋白单独表达者完全缓解率显著降低且2者之间存在相关性。3种耐药蛋白在正常对照组与初治组间表达差异有统计学意义。外周血白细胞数≥10×109/L时,LRP阳性表达率显著增高。结论:耐药蛋白表达时白血病患者预后不良,3者均与临床耐药相关。Pgp/Bcl-2 2者联合检测时较单独测定某一蛋白在评定白血病预后不良时更具有显著的临床意义。LPR对高白细胞白血病患者的耐药性和预后评定具有很高的判断价值。Objective：Through examinations of P-glycoprotein （P-gp） ,anti-apoptosis factor 2 （Bcl-2） ,lung resistance protein （LRP）, so as to show： relations between protein expression and complete remission （CR）, whether there is significant differences of protein expression between normal donors and the previously untreated patients, relationship between high white blood cell （WBC） counts of peripheral blood and high expression of drug resisrance protein. Method： lmmunohistochemistry method was performed to reveal expression of Pgp, Bcl-2, LRP. SPSS 10.0, Χ^2 analysis and linear correlation analysis were used to analyze stasistical results. Result：CR rates in patients with positive expressions of multidrug resistance proteins were lower than in those patients with negative expressions （ P 〈0.05）. CR rate in sufferers with co-expression of Pgp and Bcl-2, was significantly lower than in patients with single protein expression （ P 〈0.01）. Moreover there was correlation between Pgp expression and Bcl-2 expression, Expressions of three drug resistance proteins were significant differences between normal donors and previously untreated patients （ P 〈 0.01）. LRP expression increased obviously when WBC counts≥10 × 10^9/L （ P 〈0.05）. Conclusion： Drug resistance proteins expressions may account for poor prognosis. There were relations between them and clinical chemotherapy resistance. Co-examination of Pgp and Bcl-2 had greater significance than examination of single protein expression in accessing poor prognosis of leukemia patients. As for assessing drug resistance and prognosis of leukemia patients with high peripheral blood WBC counts, LRP had great judging value.

关 键 词：白血病 抗药性 多药 

分 类 号：R733.7[医药卫生—肿瘤]