海藻酸钠固定化木聚糖酶的研究  

Study of Xylanase Immobilized on Alginate

作　　者：王娜[1] 朱启忠[1] 任延刚[1] 张瑞景[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《北方园艺》2009年第5期92-94,共3页Northern Horticulture

摘　　要：以海藻酸钠为载体,研究了木聚糖酶的固定方法,并利用固定化酶和游离酶进行了酶解试验。结果表明:固定化酶的最适温度,热稳定性,最适pH,稳定性及与底物的亲和力都有所提高。其中采用直接包埋方式固定率低,稳定差;交联包埋法固定的固定化酶反应pH范围变宽,固定率高,但与底物的亲和能力强和操作稳定性不如包埋交联法。Xylanase was immobilized by using sodium alginate as carrier. The immobilized methods were studid. The immobilized xylanase was used to hydrolyze xylan. The results showed that the optimum temperature,thermal stability, optimal pH and pH stability of ira-mobilized xylanase had increased. And its affinity for substrate was improved. Immobilized xylanase made by direct-embedded method had low immobilized rate and bad stability. The reaction pH of immobilized xylanase using cross-linking of embedded method broadens , and the immobilized rate was high. But its affinity for substrate and operation stability were behind embedded cross-linking method.

关 键 词：木聚糖酶 海藻酸钠 固定化 

分 类 号：Q946.5[生物学—植物学]