大白菜萝卜细胞质雄性不育系RC_7花药发育的解剖学和同工酶研究  

Analyses of anatomy and isozymes in the development of microspore of CMS RC_7 in Chinese cabbage (Brassica campestris L.ssp. pekinensis)

作　　者：赵会芳[1] 巩振辉[1] 赵利民[1] 柯桂兰[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《中国农业大学学报》2009年第2期64-69,共6页Journal of China Agricultural University

基　　金：农业科技成果转化资金项目(O5EFN2l6100284);陕西省"13115"科技创新工程重大科技专项(2008ZDKG-03);陕西省科技攻关项目(2007K01-07-03)

摘　　要：采用解剖学和聚丙烯酰胺凝胶电泳技术,研究了大白菜萝卜细胞质雄性不育系RC7及其保持系细胞学特征和蕾期小孢子发育过程中过氧化物酶(POD)同工酶、过氧化氢酶(CAT)同工酶、酯酶(EST)同工酶和淀粉酶(AMY)同工酶4种同工酶酶谱的变化。同工酶分析表明,RC7不育系的POD和AMY同工酶酶谱在小孢子发育的整个时期与保持系B7都存在明显差异,且CAT和EST同工酶酶谱在花粉粒成熟时期比保持系B7少了2条清晰的谱带,酶谱差异表达时期与细胞学上观察到的败育时期一致。结果表明,单核期RC7开始败育。By means of anatomy and vertical slab polyacrylamide gel electrophoresis, cytology characteristics as well as isoenzymes of perioxidase（POD） ,catalase（CAT） ,esterase（EST） and amylase（AMY） of cytoplasmic male sterility （CMS） line RC7 and its maintainers have been analyzed. Results showed that microspore abortion occurred in the uninucleate pollen grain stage of CMS RCT. Difference of the number and intensity of the bands in POD and AMY zymograms was observed in flower buds at different stages of flower development between RC7 and B7. For CAT and EST zymogram, maintainer line B7 had two more bands than that of the CMS RC7 in the binucleate pollen stage. Expression differences of CAT and EST zymogram between RC7 and B7 occurred at the same stage as that of cytological abortion.

关 键 词：大白菜 细胞质雄性不育 同工酶 解剖学 

分 类 号：S603.2[农业科学—园艺学]