淋球菌膜抗原CppB基因的克隆及在大肠杆菌和减毒鼠伤寒沙门菌中的表达  

Cloning of the Gonococcal CppB Antigen Gene and its Expression in E. coli and Attenuated S.typhimurium

作　　者：吴卫[1] 林元凯 江琪 王磊[1] 任大明[1] 谢匡成[2] 温文清[2] 

机构地区：[1]复旦大学遗传学研究所遗传工程国家重点实验室,上海200433 [2]上海市第一人民医院中心实验室

出　　处：《上海免疫学杂志》1998年第1期8-11,共4页Shanghai Journal of Immunology

摘　　要：将PCR扩增的淋球菌CppB膜蛋白基因区段,分别克隆在游离蛋白表达载体pKK223-3和融合蛋白表达载体pWR450-1上,以点杂交证实了克隆子。控制合适表达条件,在大肠杆菌中获高效表达,免疫印迹证实其可被淋病患者血清特异识别,将融合蛋白表达质粒转入减毒鼠伤寒沙门氏菌中也获表达,为淋病口服活疫苗的制备奠定了基础。The gene of a specific antigen protein CppB from 4. 2kb crypid plasmid of Neisseria gon-orrhoeae was cloned by PCR and gene recombinant techniques. Using pure-protein-expressing vector pKK223-3 and fused-protein-expressing vector pWR450-1,the CppB gene was highly expressed in E. coli. Western blot demonstrated its immunological reaction with sera from gonorrhoea patients. Then the fused-protein-expressing plasmid pWRJD was transformed into attenuated Salmonella systems, in which the expression of the fused-protein was also detected. The results might have brought the appearance of gonococal oral live vaccines a step nearer.

关 键 词：淋球菌 CppB膜 抗原 沙门氏菌 活疫苗 

分 类 号：R378.16[医药卫生—病原生物学] R759.201[医药卫生—基础医学]