抗早幼粒白血病短型融合蛋白单克隆抗体的研制  

Preparation of Monoclonal Antibodies Against the PML-RAR α Short Type Fusion Protein

作　　者：朱军[1] 李先根[2] 缪金明[2] 钟华[2] 方智雯[2] 欧阳仁荣[2] 

机构地区：[1]上海血液学研究所分子生物学实验室,200025 [2]上海第二医科大学附属仁济医院血液科,上海200001

出　　处：《上海免疫学杂志》1998年第1期17-19,共3页Shanghai Journal of Immunology

基　　金：国家自然科学基金;卫生部基金;上海血液学研究所胡应洲基金的部分资助

摘　　要：以急性早幼粒白血病人短型融合蛋白的PML与RARα结合部位的13个氨基酸序列制备合成肽,交联甲状腺球蛋白免疫Balb/c小鼠,通过杂交瘤技术获得两株单抗,经鉴定与相关蛋白无交叉反应,说明此融合部位外露,可以制备出针对性抗体。A peptide of 13 amino acids correspoding to the short type PML-RAR a juntions was synthesized as an antigen. Peptide was coupled with thyroglobulin by MBS. By using the hybrido-ma techique we have generated two mouse monoclonal antibodies against short type PML-RARa juntion point. The isotype of both the monoclonal antibodies was IgG1 and has no cross-reaction with related protein , indicating the specificity for PML-RAR a S type fusion-point epitope.

关 键 词：融合蛋白 单壳隆抗体 急性白血病 

分 类 号：R733.704[医药卫生—肿瘤] R392－33[医药卫生—临床医学]