熊去氧胆酸对实验性大鼠淤胆保护作用的研究  

A Study on the Protective Effect of UDCA in the Model of Cholestatic Rats

作　　者：陈隆典[1,2,3,4] 冯鲁中[1,2,3,4] 倪伟虹 黄钦田 张辰官 

机构地区：[1]南京市鼓楼医院消化科 [2]南京医科大学药理学教研室 [3]南京医科大学生化教研室 [4]南京医科大学病理学教研室

出　　处：《南京医科大学学报（自然科学版）》1998年第2期132-135,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：为建立大鼠淤胆模型，并观察熊去氧胆酸（ＵＤＣＡ）对其的作用。将雌性大鼠分成４组：对照组每只灌胃０．５％羧甲基纤维素（ＣＭＣ）；模型组每只灌胃雌二醇（ＥＥ）及氯丙嗪（ＣＰＺ）；大剂量ＵＤＣＡ组每日给ＥＥ及ＣＰＺ，同时灌胃ＵＤＣＡ６０ｍｇ／ｋｇ；小剂量ＵＤＣＡ组则给ＵＤＣＡ３０ｍｇ／ｋｇ。给药１４天，分别于第７及１４天处死５及１０只，停药７天后处死剩余５只，宰杀时取血测肝功能，称空腹体重及肝重，并取鼠肝标本切片光镜检查。结果：给药１周后模型组与对照组在脏器系数与生化指标方面有显著差异，２周后差异明显加大，且与ＵＤＣＡ剂量相关。本实验以ＥＥ及ＣＰＺ制成的大鼠淤胆肝损模型可用于临床肝内淤胆性疾病的基础研究；ＵＤＣＡ使大鼠免受肝毒性药物的攻击。In order to establish the model of cholestatic rats and observe the effect of UDCA on the model, we divided the female rats at random into 4 groups. A, control, 0.5% CMC 1 ml/100 mg b.w/d orally; B, model, EE 5 mg/kg.b.w./d+CPZ 30 mg/kg.b.w./d orally; C, UDCA (60 mg/kg.b.w./d) treated the model rats orally; D, UDCA (30 mg/kg.b.w./d)treated the model rats. Discontinued all the drugs on day 14. On day 7 and day 14 killed 5 and 10 rats respectively, then on day 21 killed the rest. On the killing day, assay T Bili, ALT, ALP, γGT; Measure the body and liver weight,calculate the organ coefficient (OC); Take the liver sample for hsitological study. Results: significant difference observed between the control and the model on OC and biochemical assay on day 7 ( P <0.01); The two UDCA treated groups were different significantly with the model on OE and ALT on day 7 ( P <0.05); On day 14, the difference even more significant between the model and the two treated groups ( P <0.01), and it is related with the UDCA doses. On day 21, the difference still showed between the model and the other groups. Conclusion: Established cholestatic rats with EE and CPZ can be used for clinical intrahepatic cholestasis research; UDCA can protect rat liver from the attack of hepatic toxic drugs.

关 键 词：熊去氧胆酸 胆汁淤积 保护作用 

分 类 号：R575.630.5[医药卫生—消化系统]