EB病毒BHRF1基因抗辐射作用的研究  

The study of radioresistance of BHRF1 gene

作　　者：黄海[1] 潘星华[2] 孔宪涛[3] 余龙[4] 周水淼[1] 李兆基[1] 温武[1] 傅强[4] 孙喜元 

机构地区：[1]第二军医大学附属长海医院耳鼻咽喉科,上海200433 [2]第二军医大学生物学教研室 [3]第二军医大学附属长征医院全军临床免疫中心 [4]复旦大学遗传学研究所基因工程国家重点实验室

出　　处：《临床耳鼻咽喉科杂志》1998年第3期131-133,共3页Journal of Clinical Otorhinolaryngology

基　　金：全军医药卫生基金!960060

摘　　要：为了解EB病毒编码基因BHRF1的表达对鼻咽癌细胞抵抗放射线损伤能力的改变，构建携有BHRF1的高表达载体，并转染鼻咽癌细胞株CNE2细胞，观察转染前后的癌细胞在放射线照射后于裸鼠体内成瘤能力改变的情况。结果发现，在放射线照射前，BHRF1表达细胞在裸鼠体内形成肿瘤时间较晚，肿瘤重量较轻（P＜0．01）；而经放射线照射后，BHRF1表达细胞在裸鼠体内的成瘤能力强于对照组（P＜0．01），经原位末端标记检查发现其凋亡指数小于对照组（P＜0．01）。提示BHRF1的表达能通过阻止细胞的凋亡，增强鼻咽癌细胞抵抗放射线对其DNA的损伤作用。In order to learn relationship between the expression of BHRF1 gene and radiative sensitivity of cells of nasopharyngeal carcinoma. High BHRF1 expressive vector was set up and transfected in the cell line of nasopharyngeal carcinoma, CNE2. Then the cells were radiated to test its ability of clone formation in nude mice. The results showed that the tumors formed by BHRF1-CNE2 cells were lighter before radiation and weighter after radiation (P <0.01), and apoptotic index of BHRF1-CHE2 was less than control groups (P <0.01). So, it suggests that the BHRF1 expression could prevent apoptosis by enhancing the resistibility of the tumor cells to radiation.

关 键 词：EB病毒 BHRF1基因 鼻咽肿瘤 放射疗法 抗辐射 

分 类 号：R739.630.5[医药卫生—肿瘤] R373.9[医药卫生—临床医学]