FTIR用于第4代太空诱变育种黄芩的分析  

Analysis of the 4th Generation Scutellaria Baicalensis Georgi with Space Mutagenesis Via FTIR Spectroscopy

作　　者：丁喜峰[1] 高华娜[1] 郭西华[1] 王志宙[1] 杨腊虎[2] 关颖[1] 史锦珊[3] 

机构地区：[1]燕山大学理学院,河北秦皇岛066004 [2]中国药品生物制品检定所,北京100050 [3]燕山大学电气工程学院,河北秦皇岛066004

出　　处：《光谱学与光谱分析》2009年第5期1286-1288,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50575193);河北省教育厅教改项目(Y008)资助

摘　　要：首次从整体上测定并对比分析了我国独创的太空诱变育种黄芩和地面组黄芩,以期为全面了解第4代太空诱变黄芩品质的变化和深入研究积累资料。结果表明两组样品的FTIR光谱的主要吸收峰的峰位、峰形大致相同,但吸收峰的强度却有明显差异,太空组黄芩中各主要吸收峰强度都比地面组明显增强,尤其是黄芩的主要活性成分黄酮类化合物(黄芩甙、黄芩素、汉黄芩甙、汉黄芩素及黄芩新素等)在3391,1655,1069cm-1等处吸收峰强度明显比地面组增强,说明太空组黄芩中主要活性成分黄酮类化合物及其他成分含量比地面组明显提高。这表明太空组黄芩的非晶态活性成分明显得到了优化。太空诱变育种有利于选育出活性成分提高的黄芩新品种,是中药材种质资源创新的快捷有效的途径之一。Determination and analysis for the Scutellaria baicalensis Georgi with space mutagenesis and the ground group were carried out with FTIR for the first time in order to fully understand the quality changes of the 4th generation of space mutagenesis of Scutellaria baicalensis Georgi and do further research. The result shows that for the FTIR spectra of the two samples the position and shape of main absorption peaks are approximately the same, indicating that the major components and the structures of space group remain intact, but the intensities of the absorption peaks are obviously different, with the intensities of the space group significantly enhanced compared to the ground group, especially for the flavonoid compounds （baicalin, baiealein, wogonoside, wogonin and wogonoside etc）, which are the main active components of the Scutellaria baicalensis Georgi, the absorption peak intensities at 3 391, 1 655 and 1 069 cm^-1 are obviously stronger than those of the ground group, so the contents of flavonoid compounds and other components are higher, and the amorphous active components are optimized. Space mutation breeding is conducive to breeding new varieties of highly active components, and it is also one of the ways to innovate Chinese medicines germplasm resources efficiently.

关 键 词：太空诱变育种 黄芩 傅里叶变换红外光谱法(FTIR) 

分 类 号：O657.3[理学—分析化学]