内源性BDNF促进受损神经系统再生的实验研究  

Endogenous BDNF Promote Regeneration of Injured Nervous System

作　　者：巴迎春[1] 段艳萍[2] 王金德[1] 李云玫[3] 李明[1] 王廷华[4] 

机构地区：[1]昆明医学院解剖学教研室,昆明650031 [2]昆明医学院组织胚胎学教研室,昆明650031 [3]成都军区昆明总医院神经内科 [4]昆明医学院神经科学研究所,昆明650031

出　　处：《四川大学学报（医学版）》2009年第3期418-421,共4页Journal of Sichuan University(Medical Sciences)

基　　金：昆明医学院博士创新基金(编号:KM2007D07)资助

摘　　要：目的研究内源性脑源性神经营养因子(BDNF)对受损神经系统再生的影响。方法单侧坐骨神经切断实验:将大鼠左侧坐骨神经切断,并于术毕分为两组,每组8只,一组使用BDNF抗体,另一组则为使用正常羊血清(NSS)的对照组,第8d时都行脊髓背柱横断术。将每只大鼠两侧L5的脊髓脊根节(DRG)作纵向切片,进行免疫荧光染色,计数大鼠L5DRG内的生长相关蛋白-43(GAP-43)阳性细胞。双侧坐骨神经切断实验:将大鼠双侧坐骨神经切断,然后在第8d行脊髓全横断手术(tSCI),术后分作两组,使用NSS的对照组和使用BDNF抗体组,应用辣根过氧化物酶(HRP)追踪技术和免疫组织化学ABC法来评价内源性BDNF对脊髓损伤后的修复作用。结果单侧坐骨神经切断实验的BDNF抗体组左侧L5DRG内的GAP-43阳性细胞数少于对照组左侧(P<0.01)。双侧坐骨神经切断实验的BDNF抗体组在tSCI处的HRP阳性神经纤维数较对照组减少(P<0.01)。结论内源性BDNF对受损神经系统的再生具有积极促进作用。Objective To test the effect of endogenous BDNF on injured nervous system. Methods The left sciatic nerves of the rats were cut off, and then divided into two groups, each with 8 rats. The rats in the experimental group were intraperitoneally injected with anti-BDNF, while the control group was given normal sheep serum （NSS）. The dorsal coclums of the rats were cut on the 8^th day. The L5 DRG were cut into length wise sections to dye with fluorescent antibody. Positive cells of GAP-43 in the L5 DRG were counted. The second experiment had both sides of sciatic nerves of the rats cut off, followed by the same procedure as the first experiment. The HRP tracing and ABC immunohistochemical staining were performed to analyse the effect of endogenous BDNF on the recovery of injured spinal cords. Results For the rats with left sciatic nerves cut off, the experimental group had less GAP-43 positive cells in the left L5 DRG than the controls （P〈0.01）. For the rats with both sciatic nerves cut off, the experimental group had less nerves with positive fibers-labeled HRP than the controls at the site of tSCI （P〈0.01）. Conclusion Endogenous BDNF promote the recovery of injured nervous system.

关 键 词：坐骨神经 脊髓损伤 脑源性神经营养因子 

分 类 号：R741.02[医药卫生—神经病学与精神病学] R651.2[医药卫生—临床医学]