气相色谱外标法测定环十五内酯  

Determination of Cyclopentadecanolide Synthesized from Malania oleifera Chun et S.K. Lee Kernel Oil by Gas Chromatography with External Standard Method

作　　者：赖芳[1] 刘雄民[1] 李伟光[1] 马丽[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《食品科学》2009年第10期159-161,共3页Food Science

基　　金：广西自然科学基金项目(桂科自0135013);教育部科学技术研究重点项目(204110);广西教育厅研究项目(桂教科研[2005]47号)

摘　　要：进行蒜头果油合成环十五内酯的定量分析方法研究,用气相色谱外标法建立环十五内酯的定量分析方法,以环十五内醋的峰面积(A)对应的浓度(C)进行线性回归分析,得到通过原点的回归方程为:A=2238.7C(r=0.9993),可以利用单点校正对标准曲线进行校正,回收率为95.11%～103.3%,并利用该法跟踪测定了蒜头果油合成环十五内酯产物的含量。Gas chromatography （GC） with area-external standard method was applied to quantitative analysis of cyclopentadecanolide in its synthesis from Malania oleifera Chun et S.K. Lee kernel oil. A linear regression equation was obtained, which was A = 2238.7C （r = 0.9993）, where A was the GC peak area, and C was the cyclopentadecanolide concentration, and the standard curve was validated by single-point external bracketing standard. The recoveries of cyclopentadecanolide in 3.578 mg of sample at the spiked amounts of 1.977, 3.954 and 5.931 mg were 95.11% to 103.3%, the RSDs were 1.51% to 2.19% （n = 3）. The determination results of cyclopentadecanolide content in four prepared sample solutions were 2.14 to 4.52 mg/ml, and the RSDs were 0.66% to 2.36% （n = 3）.

关 键 词：环十五内酯 气相色谱法 外标法 

分 类 号：O657.71[理学—分析化学]