药用植物五味子内生真菌的分离及其抑菌活性研究  

Study on Isolation of Endophytic Fungi From Schisandra chinensis(Turcz.)Baill. and Their Antimicrobial Activity

作　　者：郑春英[1,2] 陆欣媛[3] 王满玉 吴丹[1] 徐慧春[1] 

机构地区：[1]黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,哈尔滨150080 [2]黑龙江省嘉峰食药用菌生物工程技术研究中心,哈尔滨150070 [3]黑龙江省农业科学院耕作栽培所,哈尔滨150086 [4]黑龙江北菌生物工程有限公司,哈尔滨150070

出　　处：《中国药学杂志》2009年第9期661-664,共4页Chinese Pharmaceutical Journal

基　　金：黑龙江省自然科学基金面上项目(D200812);黑龙江省博士后科研启动金项目(LBH-Q06016);黑龙江大学杰出青年基金项目

摘　　要：目的研究药用植物五味子内生真菌及其次生代谢产物的抑菌活性,旨在获得有良好抑菌活性的菌株,以寻找新型抗菌物质。方法以大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌、短小芽孢杆菌、铜绿假单孢菌、单增李斯特菌、肺炎克雷伯菌、鲍曼不动杆菌、酿脓链球菌、屎肠球菌、粪肠球菌和白假丝酵母为试验菌株,用菌饼法及纸片法对分离的真菌及其次生代谢产物进行了抑菌实验。结果从药用植物五味子中分离到36株内生真菌,其中有33株内生真菌对指示菌均表现出不同的抑菌活性,占总分离菌株的91.67%;有30株内生真菌的发酵液对指示菌株也有一定的抑制作用,占总分离菌株的83.33%。结论药用植物五味子的内生真菌及其次生代谢产物中存在着丰富的抑菌天然活性物质,这可为寻找新型抗菌物质提供资源。OBJECTIVE To investigate the endophytic fungi from Schisandra chinensis（Turcz.）Baill, and their secondary metabolites. METHODS Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Bacillus pumilus, Pseudomonas aeruginosa, Listeria monocytogenes , Klebsiella penumoniae , Acinetobacter baumannii , Streptococcus pyogenes , Enterococcus faecium , Enterococcusfacalis and Candida albicans were selected as the indicators for human pathogenic microbes. Antimicrobial tests of the isolated endophytic fungi and their secondary metabolites were carried out by colony disc method and paper disc diffusion method. RESULTS Among 36 strains ofendophytic fungi isolated from Schisandra chinensis（Turcz.）Baill., 33 strains （91.67% of the total strains isolated） showed the different antimicrobial activity against 12 human pathogenic microbes, and the secondary metabolites of 30 strains at least （83.33% of the total strains isolated） presented the antimicrobial activity against 12 human pathogenic microbes. CONCLUSION Diverse antimicrobial natural products are existed in endophytic fungi and their secondary metabolites from Schisandra chinensis（Turcz.）Baill., which can be the resources for new antimicrobial products.

关 键 词：五味子 内生真菌 抑菌活性 

分 类 号：R931[医药卫生—生药学]