兔VX2腹膜转移癌模型的建立及转移特征研究  

Establishment and Identification of Rabbit Peritoneal Carcinomatosis VX2 Tumor Model

作　　者：梅列军[1] 杨肖军[1] 李雁[1] 杨国梁[1] 

机构地区：[1]武汉大学中南医院肿瘤科肿瘤生物学行为湖北省重点实验室,武汉市430071

出　　处：《中国肿瘤临床》2009年第9期523-526,共4页Chinese Journal of Clinical Oncology

基　　金：教育部新世纪优秀人才支持计划项目(编号:NCEF-04-0669);全国优秀博士学位论文作者专项资金资助(编号:200464)

摘　　要：目的:建立大动物腹膜转移癌模型,鉴定其生物学行为。方法:36只新西兰大白兔分为开腹包埋、开腹穿刺及直接经皮穿刺种植三组(每组12只),每组又分为6只瘤块接种,6只悬液接种,接种VX2肿瘤。观察肿瘤生长状况,通过病理学和影像学检查分析局部区域及远处转移情况。结果:三种方法构建模型的成功率分别是100%(12/12)、91.7%(11/12)和58.3%(7/12),开腹法与经皮穿刺法相比有显著性差异(P<0.05)。接种2周后,形成典型溃疡型胃癌并腹膜转移癌表现,宿主衰竭,4周出现肺转移。病理学检查符合典型VX2组织学特点。结论:开腹包埋与开腹穿刺接种制作VX2兔腹膜转移癌模型简单,实验周期短,接种率高,其病理表现类似人类腹膜转移癌,为腹膜癌治疗的实验研究提供可靠的大型动物模型。Objective： To establish a stable model of peritoneal carcinomatosis in rabbit using VX2 tumor and to analyze the features of metastasis. Methods： VX2 tumor was implanted in 36 New Zealand rabbits by 3 methods： laparotomic implantation, laparotomic puncture and percutaneous puncture （12 animals per group）. Six rabbits were subjected to cancer tissue inoculation and 6 to inoculation of a cancer cell suspension. Detailed pathological examination was conducted at 1 week after inoculation. Remdts： The success rates of peritoneal carcinomatosis model establishment through the 3 methods were 100% （12/12）for the laparotomic implantation, 91.7% （11/12） for the laparotomic puncture, and 58.3% （7/12） for the percotaneous puncture. There was no significant difference between laparotomic implantation and laparotomic puncture （P〉0.05）, but there was a significant difference between laparotomic puncture and percutaneous puncture （P〈0.05）. At 2 weeks after inoculation, gastric cancer with peritoneal carcinomatosis showed typical VX2 tumor pathology, with widespread intraperitoneal metastatic nodules, bloody ascites and perspicuous pulmonary metastasis. Conclusion： Orthotopic tumor tissue implantation to establish a peritoneal carcinomatosis model is convenient, feasible, and timesaving. The clinicopathological features of the model are similar to those of peritoneal carcinomatosis.

关 键 词：腹膜转移癌 VX2肿瘤 动物模型 

分 类 号：R735.2[医药卫生—肿瘤]