siRNA沉默AnnexinⅡ对淋巴瘤细胞系Jurkat细胞增殖和趋化能力的影响  

Effects of Annexin Ⅱ gene silencing by siRNA on proliferation and invasive potential of Jurkat lymphoma cells

作　　者：包红雨[1] 江淼[1] 马珍妮[1] 沈飞[1] 朱明清[1] 陈琳[1] 谢丽倩[1] 董宁征[1] 阮长耿[1] 

机构地区：[1]苏州大学附属第一医院、江苏省血液研究所,卫生部血栓与止血重点实验室,215007

出　　处：《中华血液学杂志》2009年第5期303-306,共4页Chinese Journal of Hematology

基　　金：2006年度美国血液学会（ASH）血液学发展基金（ASH2006071）;江苏省“135”重点学科基金

摘　　要：目的观察沉默AnnexinⅡ（AnxA2）基因对淋巴瘤细胞系Jurkat细胞增殖、趋化能力的影响。方法应用实时荧光定量RT—PCR和流式细胞术鉴定小干扰RNA（siRNA）对人淋巴瘤细胞系Jurkat细胞的转染效果，应用MTT法检测AnxA2 siRNA对Jurkat细胞增殖的作用，应用Transwell检测对细胞趋化能力的影响。结果AnxA2 siRNA干扰组在24、48、72h细胞生长抑制率分别为（17．4±2．3）％、（22．4±3．8）％和（37．6±1．5）％，与阴性对照组[分别为（-1．3±5．1）％、（-5．5±4．4）％和（-10．8±5．5）％]比较差异有统计学意义（P〈0．05），AnxA2 siRNA干扰对Jurkat细胞增殖起抑制作用；AnxA2 siRNA干扰组细胞趋化能力（11．3±4．2）显著低于阴性对照组（54．3±8．7，P〈0．01），AnxA2 siRNA干扰可显著降低细胞趋化能力。结论siRNA沉默AnxA2基因可抑制Jurkat细胞增殖、趋化能力。Objective To investigate the effects of Annexin Ⅱ （AnxA2） gene silencing by siRNA on proliferation and invasive potential of lymphoma cell line Jurkat cells. Methods A synthesized siRNA duplex targeting to AnxA2 was transfected into Jnrkat cells. Transfection efficiency was analyzed by real-time PCR and flow cytometry. MTT assay for cell proliferation and transwell plates for invasive potential were performed. Results Compared with the negative controls, the cell proliferation inhibitory rate of the AnxA2 siRNA transfected Jurkat cells was significantly increased at 24 h, 48 h and 72 h [ （ 17.4 ± 2.3 ） % , （22.4 ± 3.8 ） % , （37.6±1.5）% vs （ -1.3±5.1）%, （ -5.5±4.4）%, （ -10.8± 5.5）%, respectively, P〈0.05]. The cell invasive potential of the transfeeted Jurkat cells was inhibited remarkably at 48 h （ 11.3 ± 4.2 vs 54.3 ± 8.7, P 〈 0.01 ）. Conclusion AnxA2 gene silenced by siRNA can inhibit the proliferation and the invasive potential of Jurkat cells remarkably.

关 键 词：RNA干扰 基因 AnnexinⅡ JURKAT细胞 细胞增殖 趋化作用 

分 类 号：R686[医药卫生—骨科学]