高温纤维素分解菌的初步分离筛选  

Isolation and screening of thermophilic cellulytic organisms

作　　者：邱成书[1] 郭亚力[1] 李河[1] 

机构地区：[1]红河学院生科院,蒙自661100

出　　处：《食品科技》2009年第5期25-27,共3页Food Science and Technology

基　　金：云南省教育厅科学研究基金项目(06Y088B);红河学院A类校级重点建设学科生物化学与分子生物学(071010)

摘　　要：以滤纸条为唯一碳源，用羧甲基纤维素钠（CMC—Na）-刚果红培养基从温泉、堆肥等高温环境采集的样品中分离和筛选出3株高温纤维素分解菌（thermophilic cellose-decomposing microorganism TCM）。碳源、温度实验表明：所得的菌株在高温下具有较强的降解纤维素能力；它们能在40—60℃生长，最适生长温度为50～60℃。纤维素酶活性实验表明：筛选得到菌株具有较高的CMCase和滤纸酶活性。Specimens were collected from hot spring and compost. Tree strains of TCM were isolated through paper as the sole carbon source and Congo Red CMC-Na medium. The experiment of carbon source and temperature was done. The result showed strains of TCM had high-activity cellulose-degradation under high temperature. They could grow between 40-60 ℃ with the optimum growth temperature of 50-60 ℃. All the three strains have high activity of CMC enzyme and FPA enzyme.

关 键 词：高温 纤维素分解菌 分离筛选 

分 类 号：TS201.3[轻工技术与工程—食品科学]