PCR-变性高效液相色谱技术检测食品中产气荚膜梭菌  

Detection of clostridium perfringens by pcr-denaturing high-performance liquid chromatography in food

作　　者：郑秋月[1,2] 傅俊范[1] 徐君怡[2] 孙哲平[2] 马惠蕊[2] 曹际娟[2] 

机构地区：[1]沈阳农业大学,沈阳110161 [2]辽宁出入境检验检疫局,大连116001

出　　处：《食品科技》2009年第5期277-280,共4页Food Science and Technology

基　　金：国家“十一五”科技支撑计划课题(2006BAK02A13)

摘　　要：目的:进行PCR结合DHPLC技术,快速检测食品中污染的产气荚膜梭菌的新方法研究。方法:采用DHPLC技术检测产气荚膜梭菌特异性PCR扩增产物,优化分析条件,并探讨其灵敏度、特异性和精密度。结果:建立产气荚膜梭菌PCR-DHPLC样品吸收特征峰型图,样本检测的峰型图可用于定性或定量监测食品中产气荚膜梭菌的动态变化。结论:研究结果表明,所建立的食品中产气荚膜梭菌PCR-DHPLC方法具有特异性强、灵敏度高、快速等优点。Using PCR and Denaturing High-Performance Liquid Chromatography to identify clostridium perfringens rapidly. Detection of the specific PCR products by DHPLC under optimal conditions,discussion on sensitivity, specific and precision. Obtained the features peak of clostridium perfringens by PCR-DHPLC. The features peak can be used in dynamic monitoring of clostridium perfringenss in food qualitatively or quantitatively. Our results showed that the method of PCR-DHPLC was rapid, specific, sensitive and quickly.

关 键 词：聚合酶链式反应 变性高效液相色谱法 产气荚膜梭菌 β-毒素基因. 

分 类 号：TS201.3[轻工技术与工程—食品科学]