乳腺癌组织中端粒酶逆转录酶的表达及新辅助化疗后的变化  

Change expressing of hTERT gene in breast cancer tissue before and after neo-adjuvant chemotherapy

作　　者：周洪伟[1,2] 曾晓华[1] 刘长安[1] 邓建川[1] 

机构地区：[1]重庆医科大学附属第二医院普外科,重庆400010 [2]苏州大学附属第四医院肿瘤外科,江苏苏州214062

出　　处：《中国癌症杂志》2009年第4期262-265,共4页China Oncology

摘　　要：背景与目的:乳腺癌组织端粒酶逆转录酶(hTERT)在乳腺癌患者新辅助化疗中的作用仍不清楚。本研究探讨新辅助化疗对hTERT表达的影响。方法:2004年2月—2007年6月对53例可手术女性乳腺癌患者采用CEF方案新辅助化疗3个周期,采用RT-PCR和免疫组化法检测化疗前、后乳腺癌组织中hTERT的mRNA和蛋白表达变化。结果:新辅助化疗前癌组织中hTERTmRNA和蛋白的阳性表达率分别为77.4%、73.6%,两者间差异无显著性(P=0.791),而采用Spearman相关检验两者呈显著正相关(r=0.289,P=0.036)。新辅助化疗后hTERTmRNA和蛋白阳性表达率(28.3%,22.6%)均显著低于新辅助化疗前。hTERTmRNA及蛋白阳性表达的患者新辅助化疗有效率显著低于hTERTmRNA及蛋白阴性表达者(P值均<0.05)。结论:新辅助化疗后hTERT基因明显下调;hTERT阴性表达患者新辅助化疗疗效更明显,可作为预测新辅助化疗疗效的一个重要参考指标。Background and purpose： hTERT is indistinct in the etlect otneo-adjuvant claemotnerapy, uur aim was to detect both expression of hTERT mRNA and protein in breast cancer tissue and their changes after neo- adjuvant chemotherapy. Methods： From Feb 2004 to Jun 2007, 53 cases with untreated advanced breast cancer were enrolled. All patients received CEF chemotherapy three cycles. The breast cancer tissue was collected before and after chemotherapy, hTERT mRNA and protein expression were detected by RT-PCR and immunohistoehemistry techniques. Results： The positive hTERT mRNA and protein expression in breast cancer tissue before chemotherapy were 77.4% and 73.6% respectively, and after neo-adjuvant chemotherapy were 28.3%,22.6% respectively.The effective rate of the neo-adjuvant chemotherapy of the patients with positive of hTERT expression was higher than those with negative hTERT expression. Conclusion： The chemo-agents altered hTERT gene expression, improved negative rate of hTERT expression. Detecting expression of hTERT gene in breast cancer tissue would be an important index for predicting the effects of neo-adjuvant chemotherapy.

关 键 词：乳腺癌 端粒酶逆转录酶 新辅助化疗 

分 类 号：R73-361[医药卫生—肿瘤]