蜜蜂丙糖磷酸异构酶基因及其编码蛋白的生物信息学分析及分子进化研究  

Bioinformatics Analysis and Molecular Evolution of Triosephosphate Isomerase (TPI) Gene and Protein in Apis mellifera

作　　者：徐剑[1] 周君[1] 刘晓红[1] 陆小平[1] 

机构地区：[1]苏州大学金螳螂建筑与城市环境学院,江苏苏州215123

出　　处：《江苏农业学报》2009年第2期270-275,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30671589)

摘　　要：运用生物信息学的方法对蜜蜂丙糖磷酸异构酶(AMTPI)基因及其编码蛋白序列进行了初步分析,得到了与基因和蛋白质组学研究相关的一些基本参数。结果表明,AMTPI基因序列由744个碱基组成,编码由247个氨基酸组成的蛋白质。AMTPI总的原子数目为3818,分子式为C1 207H1920N328O359S4,分子量为26898.71,等电点pI=8.04,摩尔消光系数为34950,在哺乳动物体内的半衰期为30 h。疏水性分析结果表明AMTPI为亲水蛋白,不稳定参数为27.32,属较稳定蛋白,不含信号肽序列,跨膜结构不明显,亚细胞定位于细胞质。AMTPI具有多种二级结构形式,是典型的(α/β)8结构,在SWISS-MODEL服务器三维建模后,运用ViewerLite 5.0进行序列编辑,获得了AMTPI的三级结构模型。多重比对结果显示,多个物种丙糖磷酸异构酶(TPI)的整体相似度较高,有多个保守结构,可以作为分子进化研究的材料。用MEGA4.0软件按Minimum evolution方案进行分子系统演化分析,获得了TPI氨基酸序列的分子进化树。To analyze and predict the physical and chemical characteristics of Apis mellifera triosephosphate isomerase （AMTPI）,several intergrated bioinformatics databases and local programs were used frequently. AMTPI is 744 bp in length with an open reading frame encoding protein with 247 amino acid. Total number of atoms is 3 818 and the structural formula is C1207 H1920N328 O359 S4 which molecular weight is 26 898.71. The predicted theoretical pl is 8.04 and extinction coefficient is 34 950. Estimated half-life is 30 h in mammalian reticulocytes. AMTPI is a stable hydrophilic protein located in cytoplasm. The classical （α/β）s TIM-barrel fold was established by the software ViewerLite 5.0 after 3-D structural modeling at SWISS-MODEL website. The alignment analysis showed high positional identities in several conserved regions. Triosephosphate isomerase （TPI） is a useful model for studying the enzyme degradation and a credible phylogenetic tree was constructed based on minimum evdution method in MEGA 4.

关 键 词：蜜蜂 丙糖磷酸异构酶 生物信息学分析 分子进化 

分 类 号：Q811.4[生物学—生物工程]