原发性肝癌转移与ras p53基因及其蛋白表达的关系  

Relationship between ras p53 gene RNA and protein expression and HCC metastasis

作　　者：郑世曦[1,2] 刘丽江[1,2] 邵永胜 郑清平[1,2] 阮幼冰[1,2] 武忠弼 

机构地区：[1]同济医科大学实验医学研究中心超微病理研究室 [2]武汉职工医学院

出　　处：《华人消化杂志》1998年第2期104-105,共2页

基　　金：国家自然科学基金

摘　　要：目的了解ｒａｓ，ｐ５３基因及其蛋白表达与原发性肝细胞癌（ＨＣＣ）转移的关系．方法应用免疫组化、狭缝杂交分别对１４例ＨＣＣ有转移者和１６例无转移者癌组织中ｒａｓ，ｐ５３基因蛋白和ＲＮＡ表达进行了研究．结果经微波修复抗原后，在１４例有转移ＨＣＣ组织中，Ｐ２１和Ｐ５３蛋白的阳性例数分别为１１例和１２例，在１６例无转移ＨＣＣ组织中，其阳性例数分别为５例和７例，两组间具有极显著性差异（Ｐ值分别为００１４和００２６）．ＨＣＣ有转移组ＮｒａｓＲＮＡ为无转移组的３～５倍，而两组间Ｐ５３ＲＮＡ量则几乎相等．结论经微波修复抗原后，Ｐ２１和Ｐ５３蛋白的免疫组化染色可作为衡量ＨＣＣ转移潜能的指标．IM To understand the relationship between the expression of both ras and p53 gene RNA and protein and the metastasis of primary hepatocellular carcinoma (HCC).METHODS Immunohistochemistry and slot blot were used to study ras and p53 gene protein and RNA in 14 cases of HCC with metastasis and 16 cases of HCC without metastasis respectively.RESULTS After microwave treatment, the positive rates of both p21 and p53 protein in 14 cases of HCC with metastasis were significantly higher than that without metastasis (11/14 vs 5/16, P=0014 and 12/14 vs 7/16, P=0026, respectively). The results of slot blot showed that OD value of Nras RNA in HCC with metastasis was 3 to 5 times higher than that in HCC without metastasis, whereas OD value of p53 RNA had no difference between the two groups.CONCLUSION Immunohistochemistry of p21 and p53 protein after antigen retrieval is a useful index for patients with HCC metastatic potential

关 键 词：P53蛋白 肝肿瘤 病理学 肿瘤转移 

分 类 号：R735.702[医药卫生—肿瘤]