肿瘤坏死因子α与人肝纤维化关系的研究  

Relationship between tumor necrosis factorα and liver fibrosis

作　　者：王新[1] 陈岳祥[1] 许才绂[1] 赵国宁[1] 黄裕新[1] 王庆莉[1] 

机构地区：[1]第四军医大学唐都医院消化科

出　　处：《华人消化杂志》1998年第2期106-108,共3页

基　　金：国家自然科学基金

摘　　要：目的探讨肿瘤坏死因子α（ＴＮＦα）与肝纤维化的关系．方法采用放射免疫分析法测定慢性轻型肝炎（ＭＣＨ）２０例，慢性重型肝炎（ＳＣＨ）２０例，肝硬变（ＬＣ）５１例患者及正常人３２例血清ＴＮＦα，层粘连蛋白（ＬＮ）和透明质酸（ＨＡ）含量，分析它们的变化规律．同时对其中３２例（正常人７例，ＭＣＨ３例，ＳＣＨ５例和ＬＣ１７例）肝组织中ＴＮＦα和Ⅲ型胶原用免疫组织化学方法检测并用ＶＧ染色观察总胶原含量．结果ＳＣＨ和ＬＣ患者血清ＴＮＦα，ＬＮ和ＨＡ含量均明显高于对照组（ＳＣＨ：１１１±０５９，１３０７±１７２，２１９１±１２１３；ＬＣ：０９２±０６６，１５６８±３１７，４００５±１８３７，Ｐ＜００５～００１）．在ＭＣＨ、ＳＣＨ和ＬＣ的ＬＮ和ＨＡ含量逐渐升高，并呈显著正相关（ｎ＝９１，γ＝０３１４９；Ｐ＜００１）．ＭＣＨ和ＳＣＨ患者，血清ＴＮＦα与其ＬＮ，ＨＡ含量呈正相关（ＬＮ：ｎ＝４０，γ＝０３４０４，Ｐ＜００５；ＨＡ：ｎ＝４０，γ＝０３８４７；Ｐ＜００５）．ＭＣＨ，ＳＣＨ和ＬＣ肝组织中Ⅲ型胶原及总胶原含量逐渐增加．ＳＣＨ，ＬＣ肝组织中ＴＮＦα阳性细胞数均明显高于对照组?IM To investigate the relationship between tumor necrosis factorα and liver fibrosis in patients with chronic liver disease.METHODS Radioimmunoassay was made in 20 patients with mild chronic hepatitis (MCH), 20 patients with severe chronic hepatitis (SCH), 51 patients with liver cirrhosis (LC) and 32 normal persons to determine the contents of tumor necrosis factorα (TNFα), laminin (LN) and hyaluronate (HA) in serum. The changes in and relationship between TNFα, LN and HA were analyzed. The TNFα and collagen Ⅲ were determined using immunohistochemical studies in liver tissues from 32 persons including 7 normal persons, 3 patients with MCH, 5 patients with SCH and 17 with LC.RESULTS TNFα, LN and HA levels in serum of SCH and LC patients were significantly higher than those in healthy controls (SCH: 111±059,1307±172,2191±1213; LC:092±066,1568±317,4005±1837, P<005-001), which increased gradually, and correlated positively with each other in all patients with liver diseases (n=91,γ=03149,P<001). TNFα contents showed a remarkably positive correlation with HA and LN levels in MCH and SCH (LN: n=40,γ=03404, P<005;HA:n=40, γ=03847,P<005). The total collagen content of MCH, SCH and LC increased gradually in liver biopsy specimens. The number of TNFα positive cells increased significantly in liver tissues from patients with SCH and LC (62%;45%;P<001). TNFα positive cells were mainly located in the periportal areas.CONCLUSION TNFα may be related to liver fibrosis, and might promote liver fibrosis.

关 键 词：肿瘤坏死因子 层粘连蛋白 透明质酸 肝硬变 

分 类 号：R575.202[医药卫生—消化系统]