Ⅱ型胶原酶体外分离大鼠泪腺上皮细胞及细胞培养  

Detachment of rat lacrimal gland epithelial cells with collagenase Ⅱ and cells culture in vitro

作　　者：王方[1] 彭清华[2] 陈佳文[1] 李怀凤[1] 曾志成[1] 

机构地区：[1]湖南中医药大学研究生院,湖南省长沙市410007 [2]湖南中医药大学第一附属医院中医眼科学重点学科

出　　处：《眼科新进展》2009年第5期330-332,共3页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30772824);湖南省自然科学基金资助(编号:07JJ3049);高等学校博士学科点专项基金资助(编号:200805410004)~~

摘　　要：目的探求体外分离及培养大鼠泪腺上皮细胞的最佳方法。方法用Ⅱ型胶原酶消化的方法分离泪腺上皮细胞。DMEM/F12作为培养基,辅加表皮生长因子和地塞米松,体外培养泪腺上皮细胞。结果用Ⅱ型胶原酶消化的方法获得了较纯的泪腺上皮细胞。36h后可见细胞大部分已贴壁。72h后细胞开始伸展,生长较活跃。10d后细胞基本融合。结论用Ⅱ型胶原酶消化的方法可得到较纯的泪腺上皮细胞;表皮生长因子联合地塞米松可加快细胞的生长速度。Objective To seek for the best way to detach the rat lacrimal gland （LG） epithelial cells and culture them in vitro. Methods The LG epithelial cells were obtained by collagenase Ⅱ digestion. The culture medium was DMEM/F12 supplemented with epidermal growth factor and dexamethasone. Results Pure LG cells could be obtained by this method. Most of the Cells adhered after 35 hours and began to expand after 72 hours,and grew actively. The cells coalesced primarily after 10 days. Conclusions The LG epithelial cells obtained by collagenase Ⅱ digestion are relatively pure. Epidermal growth factor combined with dexamethasone can promote the growth of the cells.

关 键 词：大鼠泪腺上皮细胞 胶原酶 细胞分离 细胞培养 

分 类 号：R777.2[医药卫生—眼科]