增生性糖尿病视网膜病变玻璃体中结缔组织生长因子的定量分析  

Quantitative analysis of connective tissue growth factor in vitreous with proliferative diabetic retinopathy

作　　者：李盛国[1] 谭湘莲[1] 李湘黔[1] 

机构地区：[1]湖南省郴州市第一人民医院眼科,423000

出　　处：《眼科新进展》2009年第5期367-369,共3页Recent Advances in Ophthalmology

摘　　要：目的定量测定结缔组织生长因子(connective tissue growth factor,CTGF)在增生性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)患者玻璃体中的浓度,探讨其在PDR发病机制中的作用。方法采用双抗体夹心酶联免疫吸附测定法定量检测27眼PDR、5眼非增生性糖尿病视网膜病变及5眼正常对照组玻璃体中CTGF的浓度。结果PDR组玻璃体中CTGF浓度(567.09±181.15)ng·L-1明显大于对照组(128.06±57.28)ng·L-1及非增生性糖尿病视网膜病变组(150.70±52.39)ng·L-1(P均<0.01)。PDR组中Ⅵ期患者玻璃体中CTGF浓度(706.17±88.78)ng·L-1大于Ⅳ期(349.20±110.60)ng·L-1及Ⅴ期(526.70±122.50)ng·L-1(P均<0.01)。结论PDR患者玻璃体中CTGF浓度升高可能与视网膜新生血管纤维膜的形成有关,CTGF在PDR发展过程中起着一定作用。Objective To determine the levels of connective tissue growth factor （CTGF） in vitreous of patients with proliferative diabetic retinopathy（ PDR）, and to explore its significance in pathogenesis of PDR. Methods Double antibody sandwich enzyme-linked immunosorbent assay was used to measure the mass concentration of CTGF in vitreous of 27 PDR eyes, 5 nonproliferative diabetic retinopathy （NPDR） eyes and 5 normal eyes. Results CTGF concentration in vitreous of PDR patients was （567.09 ± 181. 15 ）ng ·L^-1, which was significantly higher than that in normal control group（ 128.06 ± 57.28 ） ng·L^-1 and NPDR group （ 150.70 ± 52.39 ）ng·L^-1 （ both P 〈 0. 01 ）. CTGF concentration in vitreous of PDR was significantly higher in stage Ⅵ （706. 17 ± 88.78 ） ng · L^- 1 than that in stage Ⅳ （ 349.20 ± 110.60 ） ng·L^-1 and stage Ⅴ （526.70 ±122.50）ng ·L^-1 （both P〈0.01）. Conclusion Higher level of CTGF may be related to the formation of fibrous membranes of retinal new vessel in PDR, CTGF may play some roles in development of PDR.

关 键 词：增生性糖尿病视网膜病变 结缔组织生长因子 酶联免疫吸附测定法 

分 类 号：R774.1[医药卫生—眼科]