硬粒小麦-节节麦人工合成种的高分子量谷蛋白亚基组成及表达分析  

Subunits Composition and Expression of High Molecular Weight Glutenin in Triticum durum-Aegilops tau schii Synthetic Hexaploid Wheat

作　　者：蒋云[1] 原红军[2] 王益[3] 宣朴[1] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066 [2]西藏自治区农牧科学院,西藏拉萨850032 [3]四川农业大学小麦研究所,四川都江堰611830

出　　处：《西南农业学报》2009年第2期268-270,共3页Southwest China Journal of Agricultural Sciences

基　　金：教育部创新团队发展计划(IRT0453)

摘　　要：用十二烷基硫酸钠-聚丙稀酰胺凝胶电泳方法(SDS-PAGE),分析了4份天然加倍形成的硬粒小麦-节节麦人工合成种的高分子量谷蛋白亚基(HMW-GS)组成。结果表明:4份硬粒小麦-节节麦人工合成双二倍体SHW-Z1,SHW-Z2,SHW-Z3,SHW-Z4的HMW-GS分别为6+8、5+12;6+8、5+10;6+8、5+12和6+8、5+10。其中,6+8亚基来自硬粒小麦;5+12,5+10亚基分别来自节节麦As60与As65。硬粒小麦和节节麦的HMW-GS呈共显性遗传,在合成六倍体小麦背景中均得到了表达,且没有出现变异。表明可通过节节麦与二粒小麦杂交并天然加倍的途径创造桥梁种质,将节节麦的优质HMW-GS基因引入普通小麦。High molecular weight glutenin subunits among 4 samples of spontaneous doubling hexaploid wheat derived from the cross between T. durum and Ae. tauschii accessions were investigated by SDS-PAGE analysis. The results indicated that subunits of 6 ＋ 8,5 ＋ 12 were found in SHW-Zland SHW-Z3 ; subunits of 6 ＋ 8,5 ＋ 10 were found in SHW-Z2and SHW-Z4. Subnnits of 6 ＋ 8 come from T. durum. Subunits of 5 ＋ 12,5 ＋ 10 from As60 and As65. The HMW-GS of Ae. tauschii and T. durum could express in synthesized hexaploid wheat. The results showed that excellent gene from Ae. tauschii could be transferred into common wheat by spontaneous doubling hexaploid wheat derived from T. durum-Ae, tauschii hybrid.

关 键 词：HMW-GS 节节麦 合成六倍体小麦 品质育种 

分 类 号：S512.19[农业科学—作物学]