姜黄素对肝纤维化大鼠肝组织中NF-кB及ERK-1 mRNA表达的影响  

Expressions of curcumin on NF-кB and ERK-1 mRNA in rats with liver fibrosis

作　　者：胡泰洪[1] 黄利华[1] 徐敏[2] 徐微[1] 杨玲[2] 朱锐[2] 

机构地区：[1]无锡市传染病医院中西医结合肝病科,江苏无锡214005 [2]华中科技大学同济医学院附属协和医院

出　　处：《胃肠病学和肝病学杂志》2009年第5期400-403,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：江苏省中医药管理局资助(H05096)

摘　　要：目的探讨核转录因子-кB(nuclear factor kappa B,NF-кB)及细胞外信号调节激酶(extracegular signal-reglulated kinase,ERK)mRNA在CCl4致实验性肝纤维化大鼠肝组织中的表达及姜黄素(curcumin,CUR)对它们的影响。方法建立CCl4致大鼠实验性肝纤维化的模型,用免疫组化法比较正常组、模型组及CUR组的α平滑肌肌动蛋白(α-SMA)的表达,用RT-PCR法比较各组肝组织NF-кB及ERK-1 mRNA的表达。结果模型组中,ERK-1及NF-кB mRNA的表达均增高,而CUR组α平滑肌肌动蛋白则较模型组低,NF-кB及ERK-1 mRNA的表达也降低。结论CUR可能通过抑制α-SMA的表达,减弱NF-кB诱导的肝星状细胞的活化及抑制ERK-1促肝星状细胞的增殖,从而产生抗四氯化碳诱导的大鼠肝纤维化的作用。Objective To evaluate expressions of NF-κB and ERK-1 mRNA in rats with liver fibrosis and the influence of curcumin to them. Methods Liver fibrosis rats were induced by carbon tetrachloride （ CCl4 ）. Rats were divided into three groups： normal group, model group and curcumin group. The expression of alpha-Smooth Muscle Action was detected by immunohistochemistry. The expressions of nuclear factor kappaB （NF-κB） and extracellular signal-reg- ulated kinase （ERK-1） mRNA were determined by RT-PCR. Results The expression of alpha-Smooth Muscle Action was significantly lower in the rats treated with curcumin than that in the model group （ P 〈 0.05 ）. The expressions of NF-κB and ERK-1 mRNA were significantly higher in model group, but obviously lower in curcumin group （P 〈 0.05）. Conclusion Curcumin is able to inhibit the activation of hepatic stellate cell through suppressing the expression of alpha-Smooth Muscle Action and expressions of NF-κB mRNA, and it also can inhibit the proliferation of hepatic stellate cell through suppressing the expression of ERK-1 mRNA.

关 键 词：姜黄素 NF-κB ERK-1 肝纤维化 

分 类 号：R575[医药卫生—消化系统]