Livin、Caspase-3和Bcl-2在非小细胞肺癌的表达及生存分析  

Expressions of Livin,Caspase-3,Bcl-2 in Lung Tissue of NSCLC and their Prognostic Significances

作　　者：郑春晨[1] 陈愉生[1] 陈小岩[2] 李鸿茹[3] 

机构地区：[1]福建医科大学省立临床学院呼吸内科,福建福州350004 [2]福建省立医院病理科,福建福州350001 [3]福建省立医院呼吸内科,福建福州350001

出　　处：《中国呼吸与危重监护杂志》2009年第3期224-228,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：福建省自然科学基金(编号:2008J0074)

摘　　要：目的探讨Livin在非小细胞肺癌(NSCLC)中的表达,及与NSCLC病理、生物学行为指标和预后的关系。方法采用免疫组织化学法检测87例NSCLC肿瘤组织和40例肺良性组织中Livin、Caspase-3和Bcl-2蛋白的表达情况,将其表达与NSCLC病理、生物学行为等指标进行相关分析。多因素COX回归生存分析探讨Livin、Caspase-3和Bcl-2蛋白表达水平与预后的关系。结果Livin和Bcl-2蛋白在肺癌组织中的表达明显高于在肺良性病变组织中的表达(72.41%比0.0%,74.71%比0.0%,P=0.000),Caspase-3蛋白表达则明显降低(67.82%比87.5%,P<0.05)。Livin、Caspase-3和Bcl-2表达主要位于胞浆,未见核内表达。其表达与肺癌的组织细胞类型、分化程度、淋巴结转移、TNM分期、肿瘤大小以及部位无明显相关性。在NSCLC中,Livin蛋白表达与Caspase-3和Bcl-2蛋白表达有相关性(r1=-0.260,P=0.015;r2=0.351,P=0.001)。Livin、Caspase-3和Bcl-2不是影响肺癌手术患者预后的独立预后因素。结论Livin蛋白、Bcl-2蛋白在肺癌组织中表达上调,Livin的表达与Caspase-3、Bcl-2蛋白的表达呈相关性,三者在肺癌的发生发展中可能起协同作用。Livin、Bcl-2和Caspase-3蛋白表达与患者的预后无明显关系。Objective To study the expressions of Livin, Caspase-3 and Bcl-2 in lung tissue of nonsmall cell lung cancer （NSCLC） , and their relationship with the clinicopathological features and prognosis of NSCLC. Methods The expressions of Livin, Caspase-3 and Bcl-2 proteins were evaluated by immunohistochemical method in 87 NSCLC samples and 40 lung benign tissues. The relationship of their expressions with the clinicopathological features and prognosis of NSCLC were analyzed by Spearman＇ s Rank correlation and COX Regression. Results More NSCLC tissues showed expression of Livin than lung benign tissues（72.41% vs 0.0%, P = 0.000）, and the expression of Caspase-3 was significantly decreased （67. 82% vs 87.5% , P 〈 0. 05 ）. The proteins of Livin, Caspase-3 and Bcl-2 were detected in the endochylema but none was detected in nucelus. There was no relationship between the expression of each of these proteins and the clinicopathological features of NSCLC such as histologic type, tumor differentiation, lymph node metastasis ,TNM stage, the size of tumor, and tumor site. The expression of Livin was correlated with Caspase-3 and Bcl-2 expressions （ r1 = - 0. 260, P = 0. 015 ; r2 = 0. 351, P = 0. 001 ）. Livin, Caspase-3 and Bcl-2 were not independent prognostic factors of NSCLC. Conclusions The expression of Livin and Bcl-2 are up-regulated in NSCLC. The expression of Livin is positively correlated with that of Caspase-3 and Bcl-2,they might interact with each other in the carcinogenesis and development of NSCLC. The levels of Livin, BC1-2 and Caspase-3 proteins are not independent factors affecting the prognosis of lung cancer patients.

关 键 词：非小细胞肺癌 LIVIN 半胱氨酸天冬氨酸蛋白酶3 BCL-2 预后 

分 类 号：R734.2[医药卫生—肿瘤]