E-选择素鼻黏膜耐受对大鼠局灶脑缺血再灌注损伤的影响及其机制的实验研究  

Effect of nasal mucosal toleration inducted by E-selectin on cerebral ischemia reperfusion injury in rat and its mechanism

作　　者：杨磊 苗佳音[2] 梁庆成[2] 

机构地区：[1]牡丹江市第二人民医院神经内科,黑龙江牡丹江157000 [2]哈尔滨医科大学第二临床医学院神经内科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2009年第2期130-133,136,共5页Journal of Harbin Medical University

基　　金：黑龙江省教育厅科学技术研究项目(10551150)

摘　　要：目的探讨E-选择素鼻黏膜耐受对大鼠局灶脑缺血再灌注损伤的影响及其发生的可能机制。方法采用3种处理方法,在大鼠鼻内滴入磷酸盐缓冲溶液(PBS)、E-选择素或不滴入任何药物。诱导方案结束后,建立大鼠脑缺血2 h再灌注22 h模型,用流式细胞仪检测血中CD3+CD4+T、CD3+CD8+T细胞的表达,计算机检测脑梗死体积,用逆转录-聚合酶链反应(RT-PCR)检测脑缺血再灌注组织中白细胞介素-10(IL-10)及转化生长因子-β1(TGF-β1)mRNA表达。结果加强诱导组中E-选择素组较其他2组比较,以及E-选择素的加强诱导组与单程诱导组比较均表现为CD3+CD4+T淋巴细胞表达无差別(P>0.05),CD3+CD8+T表达明显减少(P<0.05),脑梗死体积明显缩小(P<0.05),IL-10 mRNA的表达增加(P<0.05),TGF-β1 mRNA表达呈增加趋势(0.05<P<0.1)。单程诱导组中各组比较不具有统计学意义。结论通过E-选择素鼻黏膜加强诱导耐受的方法能够诱导免疫耐受的发生,释放抗炎细胞因子,明显减轻大鼠脑缺血再灌注后的损伤。Objective To study the effect of mucosal toleration inducted by E-selectin on cerebral ischemia reperfusion injury in rats and its mechanism. Methods According to the single and booster toleration schedules, phosphate buffered solution（PBS） or E-selectin instilled into each nostril of rats,or rats no drug was administrated. After the toleration schedules were fininshed, Zea-longa operation was referred to make the model of focal cerebral ischemia-reperfusion. The expressions of CD3^＋ CD4^＋T and CD3^＋ CD^8 ＋ T lymphocyte subgroup were measured with flow cytometry. The volumes of cerebral infarction were measured by computer tomography. The expressions of IL-10 and TGF-β1 mRNA in cerebral ischemia-reperfusion region were evaluated with RT-PCR. Results The numbers of CD3^＋ CD4^＋ T lymphocyte had no significant difference（P 〉0.05）and CD3^＋ CD8 ^＋T cells were obviously suppressed （P 〈 0.05）, as compared the E-selectin group with the other two groups in booster toleration group, the infarction volumes were effectively decreased （P 〈 0. 05 ）, the expression of IL-10 mRNA was significantly raised up （ P 〈 0.05 ） , the expression of TGF-β1 mRNA had a slight trend toward an inerease（P 〉0.05）. There was the same result between E-selectin booster-toleration group and single- toleration group. But the levels of CD3^＋CD4^＋ T cells ,CD3^＋ CD8^＋ T cells ,the infarction volumes ,IL-10 and TGF-β1 mRNA had no significant differences among single-toleration groups. Conclusion Mucosal booster toleration schedules to E-selectin can induce immunologic tolerance and release anti- inflammatory cytokines, the brain damage is significantly decreased after reginoal cerebral ischemia reperfusion in rats.

关 键 词：E-选择素 鼻黏膜耐受 脑缺血-再灌注损伤 

分 类 号：R743.9[医药卫生—神经病学与精神病学]