一株高效溶藻放线菌的分子生物学鉴定  

Molecular Biology Identification of Alga-lysing Actinomycete AN02

作　　者：王慧玲[1] 叶新[1] 赵以军[1] 程凯[1] 

机构地区：[1]华中师范大学城市水环境生态学湖北省重点实验室,湖北武汉430079

出　　处：《环境科学与技术》2009年第6期17-19,共3页Environmental Science & Technology

基　　金：国家自然科学基金项目(30670088);湖北省科技攻关计划招标项目(2006AA305A04)

摘　　要：实验室从土壤中分离筛选得到了一株具有高效溶藻能力的放线菌,命名为AN02。对这株具有高效溶藻效果能力的放线菌AN02分别进行了形态特征、培养特征及生理生化特性的分析,分析结果表明放线菌AN02菌株的上述几个特征与细黄链霉菌(Streptomyces microflavus)的高度一致,与此同时,聚类分析结果也同样表明,放线菌AN02菌株与细黄链霉菌(Streptomyces microflavus)的高度一致,后经过比对,发现二者的16SrDNA序列的同源性高达近100%,因此,可将溶藻放线菌AN02定名为细黄链霉菌AN02(Streptomyces microflavus AN02)。By analyzing the morphological, cultural, physiological and biochemical characteristics of the strain Actinomycete AN02 isolated from the soil by the laboratory with highly efficient alga-lysing ability, it was indicated that all these characteristics were highly accorded with those of Streptomyces microflavus. Homology analysis showed that its 16S rDNA sequence has the identity of 100% compared to Streptomyces microflavus. As a result, alga-lysing actinomycete AN02 could be named as Streptomyces microflavus AN02.

关 键 词：溶藻链霉菌AN02 分子学鉴定 16SRDNA序列 

分 类 号：X17[环境科学与工程—环境科学]