双水相与超声耦合从灯盏花中提取分离类黄酮研究  

Aqueous two-phase system coupling with ultrasonic to extraction of flavonoids from Erigeron breviscapus

作　　者：高云涛[1] 戴建辉[1] 贝玉祥[1] 郭英[1] 李世月[1] 杨耀洪[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031

出　　处：《中成药》2009年第5期700-703,共4页Chinese Traditional Patent Medicine

基　　金：云南省社会发展科技计划项目(2007B148M);云南省教育厅科学基金资助项目(06Y195C);国家民委-教育部共建民族药资源化学重点实验室开放基金项目(MJY07010)

摘　　要：目的:利用丙醇-硫酸铵双水相体系与超声耦合对灯盏花中类黄酮进行提取分离。方法:丙醇-硫酸铵双水相的形成和提取条件对类黄酮得率的影响进行了研究。结果:方法可有效地从中药中提取类黄酮,对灯盏花类黄酮的提取率为6.11%,提取物类黄酮含量为22.3%,明显高于回流提取法。结论:方法操作简便。AIM： To use propyl-alcohol ammonium sulfateaqueous aqueous two-phase separation coupling with ultrasonic extraction to extract flavonoids from Erigeron breviscapus. METHODS： The effects of the formation of aqueous two-phase and ultrasonic extraction eonditons on extraction have been investigatied. RESULTS： The extraction rates of flavonoids was 6.11% in the optimum extraction and separation conditions, and the flavonoid content in extractive was 22.3%, much higher than that of reflux extraction. CONCLUSION： The proposed method is simpl.

关 键 词：超声波提取 双水相分离 灯盏花 类黄酮 

分 类 号：R283.2[医药卫生—中药学]