抗菌肽CM4组分对K562癌细胞染色质DNA断裂作用的SCGE研究  

Studies on the Action of the CM4ABP anti K562 Cancer Cells by SCGE

作　　者：王芳[1] 张双全[1] 戴祝英[1] 

机构地区：[1]南京师范大学生物系

出　　处：《生物化学与生物物理进展》1998年第1期64-67,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金;国家教育委员会留学回国人员资助

摘　　要：单细胞凝胶电泳法（ｓｉｎｇｅｃｅｌｇｅｌｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ，ＳＣＧＥ）是一种快速，敏感的检测单个哺乳动物细胞ＤＮＡ断裂的技术，也叫彗星实验（ｃｏｍｅｔａｓｓａｙ）．此实验首次通过ＳＣＧＥ法观察抗菌肽ＣＭ４组分对人髓样白血病Ｋ５６２细胞和正常人白细胞核染色质ＤＮＡ的影响，从而进一步研究抗菌肽抗癌作用的机制．荧光显微镜观察显示经抗菌肽ＣＭ４组分处理过的Ｋ５６２癌细胞核染色质ＤＮＡ出现断裂，形成一个亮的荧光头部和彗星似的尾部，而经同样处理的正常人白细胞和未经抗菌肽处理的Ｋ５６２癌细胞核染色质ＤＮＡ未出现断裂，核完整，呈圆形．经彗星尾长分析，前者ＤＮＡ损伤率平均为７３６２％，统计学处理Ｐ＜０００１，具高度显著性差异．这表明，抗菌肽ＣＭ４对Ｋ５６２癌细胞核染色质ＤＮＡ有明显的断裂作用，而对正常人白细胞则没有断裂作用．The single cell gel electrophoresis (SCGE), also called comet assay, is a simple,rapid and sensitive biochemical technique for detecting DNA single strand breakage of the mammalian cells. In order to study the anticancer mechenism of CM4component of the antibacterial peptides (CM4ABP), the SCGE was used to observe the chromatin DNA breakage action of the K562 cancer cells comparing with the leucocytes of normal human under treating with CM4ABP.Using the fluorescent microscopy, it is observed the chromatin DNA cleavage of the K562 cancer cells treated with CM4ABP forms brighte fluorescent head and comet like tail; On the contrary, the normal human leucocytes treated and the K562 cancer cells untreated with CM4ABP show intact, round nuclei, no comet tails. The analysis of comet assay showed that the averge ratio of the K562 nuclei cleavage is 7362% (P<0001). The results showed that the CM4ABP can cause the chromatin DNA of the K562 cells damaged, but it has no normal human leucocytes.

关 键 词：单细胞凝胶电泳 抗菌肽 K563癌细胞 染色质 DNA 

分 类 号：R730.51[医药卫生—肿瘤]