促红细胞生成素对新生大鼠缺氧缺血性脑损伤的脑保护研究  

Protective Effect of Erythropoietin on Hypoxic-Ischemic Brain Damage of Neonatal Rats

作　　者：李英[1] 欧阳静萍[1] 尹浩军[2] 

机构地区：[1]武汉大学医学院病理学与病理生理学系,湖北武汉430071 [2]武警湖北省总队医院神经内科,湖北武汉430061

出　　处：《武汉大学学报（医学版）》2009年第3期314-317,共4页Medical Journal of Wuhan University

摘　　要：目的:探讨促红细胞生成素(EPO)对新生缺氧缺血性脑损伤(HIBD)大鼠的神经保护作用。方法:40只7 d龄新生大鼠随机分为HIBD模型组、EPO干预组、生理盐水对照组、假手术组,每组各10例。在建立HIBD模型后,EPO干预组立即一次性腹腔注射5 000 U/kg剂量的人基因重组促红细胞生成素(rhEPO),生理盐水对照组注射等量生理盐水;于处置24 h后用原位缺口末端标记(TUNEL)法和流式细胞计(FCM)检测受损局部神经元细胞的凋亡情况,用RT-PCR检测神经元细胞凋亡相关信号分子的改变。结果:与假手术组比较,HIBD模型组在术后24 h神经元出现Bcl-2/Bax比值倒置,神经元凋亡增加;与HIBD模型组比较,EPO干预组神经元Bcl-2表达相对增加,而Bax表达相对受抑制,Bcl-2/Bax比值升高,神经元凋亡明显减少并且增殖增加,差异有统计学意义(P<0.01)。结论:EPO可以通过提高Bcl-2/Bax比值,抑制细胞凋亡而起到神经保护作用。To observe the protective role of erythropoietin （EPO） on hypoxic-isehemie brain damage in neonatal rats. Methods： A total of 40 newborn rats aged 7 days were randomly divided into hypoxie-isehemic brain damage （HIBD） group, EPO-intervention group, normal saline control group, and sham-operated group. The rats of EPO-intervention group were injected a 5 000 U/kg dose of recombinant human erythropoietin （rhEPO） into the abdominal cavity immediately after establishing HIBD model, and the rats of normal saline control group were injected the same dose of normal saline. Apoptosis of partial damage neuron after 24 hours were measured by TUNEL method and flow cytometry （FCM） respectively. The changes of neuron apoptosis related signaling molecules were detected by RT-PCR. Results： Compared with that in sham-operated group, Bcl-2/Bax ratio neurons was inverted and apoptosis of damage neuron were increased after HIBD in model group. Compared with that in HIBD group, the increasing of Bcl-2 expression, the restraining of Bax expression and the increasing of Bcl-2/Bax ratio in EPO intervention group was detected, and the decreased neuronal apoptosis and the increased proliferation were also ob- servable significantly in EPO-intervention group too （all P〈0.01）. Conclusion： EPO could play a neuroprotective role through improving the ratio of Bcl-2/Bax and inhibiting cell apoptosis.

关 键 词：脑缺氧 脑缺血 促红细胞生成素 凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]