槲皮素联合顺铂对宫颈癌HeLa细胞增殖及凋亡的影响  

Effect of Quercetin Combined with Cisplatin on the Proliferation and Apoptosis of Cervical Cancer HeLa Cells

作　　者：杨洋[1] 张蔚[1] 黄丽琼[1] 陶璐[1] 

机构地区：[1]武汉大学人民医院妇产科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2009年第3期334-336,357,F0002,共5页Medical Journal of Wuhan University

摘　　要：目的:探讨槲皮素联合顺铂对HeLa细胞增殖及凋亡的影响。方法:不同浓度槲皮素及不同浓度槲皮素联合顺铂处理HeLa细胞24 h后,采用MTT检测细胞的增殖活性、流式细胞仪Annexin V-FITC/PI法检测细胞的凋亡情况、激光扫描共聚焦显微镜观察用PI荧光标记的细胞核的形态学变化。结果:槲皮素能抑制HeLa细胞增殖,且呈剂量依赖性;槲皮素联合顺铂与相同浓度顺铂单用处理HeLa细胞相比,前者对HeLa细胞的抑制作用更显著(P<0.01);不同浓度槲皮素与顺铂联合处理HeLa细胞与顺铂单用相比,前者显著提高了HeLa细胞的凋亡率(P<0.01)。结论:槲皮素可显著抑制HeLa细胞增殖并诱导其凋亡,槲皮素与顺铂联合应用具有一定的协同效应。To study the effect of quercetin combined with cisplatin （DDP） on the proliferation and apoptosis of cervical cancer HeLa cells. Methods： HeLa ceils were incubated in vitro with DDP, quercetin respectively at different concentrations, or quercetin ＋ DDP for 24 hours. The cell proliferation was evaluated by MTT assay, the rate of cell apoptosis was detected by flow cytometry, and the nucleolus morphologie changes were observed by laser scanning eonfocal microscope after PI staining. Results： Quercetin could inhibit the proliferation of HeLa cells in a dose-dependent manner. Compared with cisplatin group, quercetin ＋ cisplatin significantly inhibited the proliferation of Helm cells（P〈0.01） ,and increased the rate of cell apoptosis （P〈0.05）. Conclusion： Quercetin, especially combined with cisplatin, can significantly inhibit the proliferation and induce apoptosis of cervical cancer cells.

关 键 词：槲皮素 顺铂 HELA细胞 凋亡 

分 类 号：R73-34[医药卫生—肿瘤]