粘虫幼虫血淋巴脂蛋白的分离鉴定及理化特性分析  

ULTRACENTRIFUGAL ANALYSIS AND BIOCHEMICAL PROPERTIES OF LIPOPROTEIN IN HEMOLYMPH OF LARVAL ARMYWORM,MYTHIMNA SEPARATA

作　　者：关雪辰[1] 欧阳迎春[1] 王宗舜[1] 

机构地区：[1]中国科学院动物研究所

出　　处：《昆虫学报》1998年第1期27-31,共5页Acta Entomologica Sinica

基　　金：国家重点自然科学基金

摘　　要：通过利用ＮａＢｒ密度梯度超速离心对粘虫Ｍｙｔｈｉｍｎａｓｅｐａｒａｔａ幼虫的血脂蛋白进行了分离及理化特性分析。查明了幼虫血淋巴内主要是高密度脂蛋白（ＨＤＬｐ），而低密度脂蛋白（ＬＤＬｐ）的含量极低。这两种脂蛋白的密度分别为ＨＤＬｐ１．１２３ｇ／ｍＬ，ＬＤＬｐ１．０５９ｇ／ｍＬ。ＨＤＬｐ和ＬＤＬｐ蛋白含量、脂类、氨基酸的种类测定结果表明ＨＤＬｐ，ＬＤＬｐ的蛋白含量分别是２５．２μｇ／μＬ和１１μｇ／μＬ。ＨＤＬｐ水解后可获得１６种氨基酸。ＬＤＬｐ可获得１５种。氨基酸的种类比较一致，其含量变化幅度也不大。ＨＤＬｐ脂类部分占最多的是二酰基甘油酯（ＤＧ）和磷脂（ＰＣ）。其余的脂类依次是碳氢化合物（ＨＣ）和胆固醇（Ｃ）。ＬＤＬｐ主要是磷脂和碳氢化合物。昆虫脂蛋白的主要中性甘油酯是二酰基甘油酯。Two kinds of lipoproteins, low density lipoproteins (LDLp, d=1059) and high density lipoproteins (HDLp, d=1123) were isolated from the larval hemolymph of Mythimna separata by ultracentrifugal analysis. LDLp was obtained as a single protein band on polyacrylamide gel electrophoresis, however, HDLp could be separtated into several protein bands by electrophoresis. Biochemical properties of HDLp and LDLp have been analyzed. Protein contents of HDLp and LDLp were 252 μg/μL and 11 μg/μL respectively. Composition of amino acids of HDLp and LDLp were analyzed by amino acids analyser. The amino acid composition was compared with that in other insects. The major lipid component of lipoproteins from insects was found to be diglycerides (DG).

关 键 词：高密度 脂蛋白 低密度 粘虫 鉴定 理化特性 

分 类 号：Q966[生物学—昆虫学] Q969.42