肿瘤坏死因子相关凋亡诱导配体对人骨肉瘤的作用  

Function of Tumor Related Apoptosis Inducing-ligand to Human Osteosarcoma Cells

作　　者：杨彩虹[1] 陈安民[1] 曾恒[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,武汉430030

出　　处：《肿瘤防治研究》2009年第5期361-364,共4页Cancer Research on Prevention and Treatment

基　　金：科技部973课题子课题资助项目(2002CB513107)

摘　　要：目的研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人骨肉瘤的作用,以及此作用与TRAIL受体(TRAILR)在人骨肉瘤中表达的相关性。方法应用原位杂交、Western blot方法检测人骨肉瘤细胞系MG-63及新鲜骨肉瘤组织块中TRAILR的表达;将在大肠杆菌中表达的TRAIL纯化后用于MG-63及新鲜骨肉瘤组织块,同法处理人白血病细胞株Jurkat作为阳性对照。MTT法检测细胞毒作用;流式细胞术检测凋亡,光镜下观察形态学变化,计数细胞,绘制生长曲线,测定细胞倍增时间。结果人骨肉瘤中死亡受体DR4、DR5呈高表达而诱惑受体DcR1、DcR2呈低表达,但TRAIL抑制人骨肉瘤细胞增殖而不能诱导其凋亡,MG-63在TRAIL作用下变肥大且呈编织排列。结论人骨肉瘤细胞对TRAIL耐受与其诱惑受体表达无关,TRAIL能改变体外培养的人骨肉瘤细胞形态及增殖动力学。Objective To study the relationship between expression of decoy receptors and susceptibility of human osteosareoma cells to TRAIL-induced apoptosis. Methods The expression of TRAIL receptors in human osteosarcoma cell line MG-63 and fresh osteosarcoma specimens was detected by in situ hybridation and Western blot. Soluble TRAIL segment was expressed in E-coli BL21, refolded and purified. Induction of apoptosis in MG-63 and Jurkat cell was assayed by examination of morphological changes under microscope and qualified by FACS analysis. Cells were counted by Trypan blue and growth curves were drawn. The proliferative activity was assessed by cell division index. Results Although death receptors were highly expressed and decoy receptors were lowly expressed in MG-63, its apoptosis can＇t be induced by TRAIL was detected in it. MG-63 ceils became hypertrophical and were ranged in a weaving look un- der microscope. The proliferation of MG-63 was inhibited by TRAIL. Conclusion It seems no correlation between expression of decoy receptors and susceptibility of human osteosarcoma cells to TRAIL in MG-63. TRAIL inhibited the proliferation of MG-63 and changed its morphology.

关 键 词：骨肉瘤 肿瘤坏死因子相关凋亡诱导配体 诱惑受体 死亡受体 

分 类 号：R738.1[医药卫生—肿瘤]