SHP-1与SOCS3基因在成人急性白血病中的表达及其与预后的关系  

Clinical Value of the Expression of SHP-1 and SOCS3 Genes in Leukemia Cells

作　　者：罗建民[1] 杨琳[1] 李燕[1] 刘小军[1] 温树鹏[1] 王福旭[1] 张敬宇[1] 张学军[1] 杜行严[1] 姚丽[1] 董作仁[1] 

机构地区：[1]河北医科大学第二医院血液内科河北省血液病重点实验室,石家庄050000

出　　处：《肿瘤防治研究》2009年第5期384-387,共4页Cancer Research on Prevention and Treatment

基　　金：河北省自然科学基金资助项目(C2005000744);河北省科技厅指导项目(052761615)

摘　　要：目的了解SHP-1基因和SOCS3基因在白血病细胞中的表达及其对预后的影响,为进一步阐明SHP-1和SOCS3基因在白血病发病中的作用机制打下基础。方法用实时荧光定量PCR(FQ-PCR)和RT-PCR方法分别对AL患者和13例正常对照的骨髓进行SHP-1、SOCS3 mRNA水平监测。结果①正常人SHP-1 mRNA均呈阳性表达,其表达水平为3.413±2.018,ALL患者SHP-1均为阴性表达;初治、复发AL组SHP-1表达水平明显低于正常对照组(P<0.01),缓解后SHP-1 mRNA水平上升但仍低于正常对照(P<0.01)。②初治和复发AL患者SOCS3 mRNA表达水平均明显高于缓解组和正常对照组(P<0.05);缓解患者SOCS3 mRNA与正常对照相比无统计学意义(P>0.05)。③SHP-1表达阳性组缓解率(76.4%)明显高于表达阴性组(47.3%)(P<0.01)。结论SHP-1和SOCS3均参与白血病发病,但发病机制不同。SHP-1与白血病的疗效预后有关。Objective To investigate the role of SHP-1 and SOCS3 in leukemia and the effects on clinical prognosis. To furtherly clarify the effects of SHP-1 and SOCS3 gene on cell proliferation signal pathway. Methods We used FQ-PCR and RTPCR to detect the mRNA level of SHP-1 and SOCS3 in AL patients and normal control respectively. Results①The expression of SHP-1 mRNA in NC was positive（3. 413±2. 018） and it was significant lower in de novo AL than NC group （P〈0. 01）. CR was achieved and decreased again after the disease replased. The expression of SHP-1 in AML was significantly different with ALL（P〈0. 001 ）.②The mRNA level of SOCS3 in de novo and relapsed AL was obviously higher than that of normal control（P〈0. 05） ,and there was no significant defference between CR group and NC group（P〉0. 05）.③ The complete remission in SHP-1 positive patients（76. 4%） was dramatically higher than those with negative SHP-1 expression（P〈0. 01 ）. Conclusion The SHP-1 and SOCS3 both effect on the leukemia, but the mechanism is different. SHP-1 is related with the prognosis of leukemia.

关 键 词：蛋白酪氨酸磷酸酶 细胞因子信号转导抑制因子3 白血病 

分 类 号：Q786[生物学—分子生物学] R733.7[医药卫生—肿瘤]