Stat3通路相关基因在散发性大肠管状腺瘤癌变中的表达及意义  

Expression of Stat3 and Correlative Factor and Its Significance in Carcinogenesis of Sporadic Colorectal Tubular Adenoma

作　　者：吴文新[1] 刘惠民[1,2] 张祥宏[1] 

机构地区：[1]河北医科大学第二医院病理科,石家庄050017 [2]河北工程大学医学院病理教研室

出　　处：《肿瘤防治研究》2009年第5期388-390,418,共4页Cancer Research on Prevention and Treatment

基　　金：河北省自然科学基金资助项目(C2006000911);河北省邯郸市科技局资助项目(072317064-2)

摘　　要：目的探讨Stat3及CyclinD1、Bcl-xL在散发性大肠管状腺瘤癌变过程中的可能作用。方法采用免疫组化方法检测107例散发性大肠管状腺瘤异型增生及癌变组织中Stat3和CyclinD1、Bcl-xL在蛋白水平的表达情况。结果腺瘤伴异型增生组和癌变组中的Stat3蛋白的表达明显高于正常大肠黏膜组(P=0.000),且癌变组中Stat3蛋白的表达高于腺瘤伴异型增生组(P=0.003);腺瘤伴异型增生组和癌变组中CyclinD1蛋白的表达均明显高于正常大肠黏膜组(P=0.000),且癌变组中CyclinD1蛋白的表达高于腺瘤伴异型增生组(P=0.000);大肠管状腺瘤伴异型增生组和癌变组中Bcl-xL在蛋白水平的阳性表达率均明显高于正常大肠黏膜组(P=0.000),而且癌变组中Bcl-xL的表达明显高于腺瘤伴异型增生组(P=0.000);Stat3、CyclinD1和Bcl-xL均随腺瘤异型增生程度的增高呈递增趋势。Stat3与Cy-clinD1、Bcl-xL的表达存在正相关关系。结论Stat3、CyclinD1和Bcl-xL可能在散发性大肠管状腺瘤癌变过程中起一定的作用。Objective To explore the possible effect of StaG, CyclinD1 and Bcl-xL in the process of sporadic colorectal tubular adenoma. Methods The expression of StaG, CyclinD1 and Bcl-xL at protein level was studied in 107 cases of sporadic colorectal tubular adenoma with dysplasia （SCTA-D） or sporadic colorectal tubular adenoma with cancerous changes（SCTA-Ca） by immunohistochemical staining. Results The positive expression of Stag Cyelin D1 and Bcl-xL in both SCTAD and SCTA Ca was remarkably higher than that in NCM （P = 0. 000）, and the positive expression of Stag was significantly higher in SCTA-Ca than that in SCTA-D （P = 0. 003）. The positive expression of CyclinDl was obviously higher in SCTA-Ca than that in SCTA-D （P = 0. 000）. The expression of Star3, CyclinD1 and Bcl-xL at protein level was all increased with the hyperplastic degree of SCTA-D. Positive correlation could be found among the expression of Stag CyclinD1 and Bcl-xL. Conclusion Stat3,CyclinD1 and Bcl xL may involved in the process of sporadic colorectal tubular adenoma.

关 键 词：STAT3 CYCLINDL BCL-XL 大肠管状腺瘤 免疫组织化学 

分 类 号：Q786[生物学—分子生物学] R735.34[医药卫生—肿瘤]