绿茶多酚保存同种异体神经移植体的实验研究  

Experimental study on nerve allografts stored in green tea polyphenol solution

作　　者：孔凡宾[1] 葛宝丰[2] 刘兴炎[2] 陈克明[2] 王勇[2] 张晓红[2] 王娟[3] 

机构地区：[1]兰州大学第二临床医学院,甘肃兰州730000 [2]兰州军区兰州总医院骨科研究所,甘肃兰州730050 [3]兰州军区兰州总医院肌电图室,甘肃兰州730050

出　　处：《中国矫形外科杂志》2009年第10期777-781,共5页Orthopedic Journal of China

基　　金：全军"十一五"重点课题(编号:06G033)

摘　　要：[目的]研究绿茶多酚溶液保存的同种异体神经修复大鼠坐骨神经缺损的效果。[方法]48只成年雄性Wista大鼠,随机分为4组,每组12只,将坐骨神经在梨状肌孔下5mm处切除1.0cm,神经缺损分别用4种移植物桥接。A组:自体神经移植;B组:新鲜异体神经移植;C组:经冷冻处理的异体神经移植;D组:用绿茶多酚保存的异体神经移植。术后6、12周通过大体观察、电生理学检查、组织学观察、透射电镜观察、图像分析与定量学检测评价各组修复神经缺损的效果。[结果]A、D组间差异无统计学意义(P>0.05),A组、D组的各项指标均优于B组、C组(P<0.05或P<0.01)。[结论]绿茶多酚溶液保存的同种异体神经是良好的神经移植替代材料。[ Objective] To explore the outcome of repairing nerve defects with nerve allografts stored in green tea polyphenol solution. [ Method] Forth-eight male mice were randomly divided into 4 groups of nerve grafting, with 12 rats in each group. A 1.0 cm sciatic nerve segment, 5 mm away from infrapiriform foramen, was removed and repaired by 4 kinds of nerve allografts. Group A ： autografts. Group B ： allografts. Group C ： cryopreserved nerve allografts. Group D ： nerve allografts stored in green tea polyphenol solution. At 6 weeks and 12 weeks, a series of examinations were performed, including the gross appearance, electrophysiological test, histological observation ,electron microscopic study and quantitative analysis with image analysis system. [ Resuit]All the parameters of groups A and D were better than those in groups B and C（P 〈0.05 or P 〈0.01 ）. There were no statiscally significant differences between groups A and D. [ Conclusion ] The nerve allograft stored in green tea polyphenol solution is a goodsubstitute for nerve graft.

关 键 词：同种异体移植 绿茶多酚 神经再生 

分 类 号：R285.5[医药卫生—中药学]