Homologue of mammalian apolipoprotein A-II in non-mammalian vertebrates  

作　　者：Malay Choudhury Shoji Yamada Masaharu Komatsu Hideki Kishimura Seiichi Ando 

机构地区：[1]Science of Marine Resources, United Graduate School of Agricultural Science, Kagoshima University, Korimoto, Kagoshima 890-0065,Japan [2]Department of Fisheries Science, Kagoshima University, Shimoarata, Kagoshima 890-0056, Japan [3]Department of Marine Bioresources Chemistry, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate,Hokkaido 041-8611, Japan

出　　处：《Acta Biochimica et Biophysica Sinica》2009年第5期370-378,共9页生物化学与生物物理学报（英文版）

摘　　要：Although apolipoprotein with molecular weight 14 kDa （apo-14kDa） is associated with fish plasma highdensity lipoproteins （HDLs）, it remains to be determined whether apo-14 kDa is the homologue of mammalian apoA-II. We have obtained the full eDNA sequences that encode Japanese eel and rainbow trout apo-14 kDa. Homologues of Japanese eel apo-14 kDa sequence could be found in 14 fish species deposited in the DDBJ/EMBL/GenBank or TGI database. Fish apo- 14 kDa lacks propeptide and contains more internal repeats than mammalian apoA-II. Nevertheless, phylogenetic analysis allowed fish apo-14 kDa to be the homologue of mammalian apoA-II. In addition, in silico cloning of the TGI, Ensembl, or NCBI database revealed apoA-IIs in dog, chicken, green anole lizard, and African clawed frog whose sequences had not so far been available, suggesting both apoA-I and apoA-II as fundamental constituents of vertebrate HDLs.Although apolipoprotein with molecular weight 14 kDa （apo-14kDa） is associated with fish plasma highdensity lipoproteins （HDLs）, it remains to be determined whether apo-14 kDa is the homologue of mammalian apoA-II. We have obtained the full eDNA sequences that encode Japanese eel and rainbow trout apo-14 kDa. Homologues of Japanese eel apo-14 kDa sequence could be found in 14 fish species deposited in the DDBJ/EMBL/GenBank or TGI database. Fish apo- 14 kDa lacks propeptide and contains more internal repeats than mammalian apoA-II. Nevertheless, phylogenetic analysis allowed fish apo-14 kDa to be the homologue of mammalian apoA-II. In addition, in silico cloning of the TGI, Ensembl, or NCBI database revealed apoA-IIs in dog, chicken, green anole lizard, and African clawed frog whose sequences had not so far been available, suggesting both apoA-I and apoA-II as fundamental constituents of vertebrate HDLs.

关 键 词：apolipoprotein A-II-like protein Anguilla japonica Oncorhynchus mykiss phylogenetic tree PROPEPTIDE 

分 类 号：Q959.3[生物学—动物学] Q513