重组人胰岛素样生长因子1对db／db小鼠血糖的影响  

Effect of Recombinant Human Insulin-like Growth Factor 1 on the Blood Glucose of db/db Mice

作　　者：周亮[1] 崔岚[2] 梁东春[3] 

机构地区：[1]中国科学院昆明动物研究所,中国科学院研究生院,650223 [2]江苏省盐城师范学院 [3]天津医科大学内分泌研究所

出　　处：《天津医药》2009年第5期387-389,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金重点项目(项目编号:033801611)

摘　　要：目的:研究重组人胰岛素样生长因子1(rhIGF-1)对近似于人类2型糖尿病的db/db小鼠的降血糖作用。方法:正常饲养db/db及同品系的正常小鼠,定期监测血糖和血胰岛素水平。待db/db小鼠表现出明显的高血糖及高胰岛素血症后随机分为4组,每日皮下注射溶媒、胰岛素或不同剂量的rhIGF-1,共2周。首次给药后每30min测定血糖1次,观测不同干预方式的即时降糖效果。再于每次给药前测定血糖,观察2周内的变化趋势。结果:db/db小鼠饲养至8周龄时表现出明显的血糖升高及高胰岛素血症。所用剂量的胰岛素对db/db小鼠未表现出明显的降血糖作用;rhIGF-1特别是高剂量rhIGF-1的即时及短期降糖效果均较明显,可有效保护db/db小鼠。结论:rhIGF-1对db/db小鼠有较好的降血糖作用,对伴有明显胰岛素抵抗的糖尿病患者具有临床应用前景。Objective： To study blood glucose regulation effect of recombinant human insulin-like growth factor 1 （rhIGF-1） on db/db defective leptin receptor mice which acted as the animal model of type 2 diabetes. Methods： Six-week old db/db and normal mice from the same strain were fed normally. Blood glucose, serum insulin and serum IGF-1 were monitored every three days. When db/db mice showed the significant evidence of hyperglycemia and hyperinsulinemia, they were divided into four groups randomly and subjected to the treatment of subcutaneous injection of solvent, insulin and rhIGF-1 of high and low doses for two weeks. Blood glucose was measured every 30 minutes after the first administration, and the effects of regulating blood glucose with different intervention methods were observed. The levels of blood glucose before every administration were evaluated, and the trends of blood glucose were observed. Results： db/db mice were characterized by hyperglycemia and hyperinsulinemia at the eighth week. There was no significant effect of insulin on blood glucose decrease. High-dose IGF-1 could decrease the blood glucose to normal level 6 days after the treatment, and could avoid the high level of blood glucose. Conclusion： The effect of rhIGF-1 on regulating blood glucose is much better than that of insulin in db/db mice. This result could contribute to the potential of using rhIGF-1 for the treatment of diabetes with severe insulin resistance.

关 键 词：胰岛素样生长因子Ⅰ 重组 遗传 血糖 胰岛素抗药性 小鼠 

分 类 号：R394.8[医药卫生—医学遗传学] R587.101[医药卫生—基础医学]