胜利油田滩涂区石油降解菌的筛选、鉴定及其多样性分析  

Isolation,identification and diversity analysis of petroleum-degrading bacteria in Shengli Oil Field wetland soil

作　　者：韩平[1] 郑立[1] 崔志松[1] 郭秀春[1,2] 田黎[1] 

机构地区：[1]国家海洋局第一海洋研究所海洋生态中心,山东青岛266061 [2]中国海洋大学化学化工学院,山东青岛266100

出　　处：《应用生态学报》2009年第5期1202-1208,共7页Chinese Journal of Applied Ecology

基　　金：海洋公益性行业专项(200705011);国家自然科学基金项目(20602009,40776098);国家“863”计划项目(2007AA09Z435,2007AA091507,2008AA09Z407);国家海洋局第一海洋研究所基金(2008G15)共同资助.

摘　　要：基于传统的实验方法,对胜利油田滩涂区土壤中石油降解菌进行了筛选和鉴定,并利用PCR-DGGE（变性梯度凝胶电泳）技术分析了胜利油田滩涂区的菌群多样性.结果表明：由研究区土壤中筛选出13株石油降解菌,其中,6株石油降解菌的石油降解率〉90%,能降解大部分C12~C26的石油烷烃,系统发育学鉴定为Alcanivorax、Halomonas和Marinobacter,均属于-γproteobacteria分支;胜利油田滩涂区未培养优势菌有Sulfurovum、Gillisia和Arcobacter;该区优势菌群中-γproteobacteria所占比重较大,其次为α-proteobactiria、-εproteobactiria、Acti-nobacteria和Flavobacteria.The petroleum-degrading bacteria in Shengli Oil Field wetland soil were isolated and identified by traditional experiment methods, and their diversity was analyzed by PCR-DGGE （denaturing gradient gel electrophoresis）. A total of thirteen petroleum-degrading bacterial strains were isolated, among which, six strains were found to have the ability of degrading the majority of C12-C26 petroleum hydrocarbon, with a degradation rate of 〉 90%. These petroleum degraders were phylogenetiely identified as the members of Halomonas, AIcanivorax, and Marinobacter, which were all belonged to 3,-proteobacteria. The uncultured predominant bacteria in Shengli Oil Field wetland soil were of Sulfurovum, GiUisia and Arcobacter. Among the predominant bacteria, γ-proteobacteria accounted for a larger proportion, followed by ε-proteobactiria＇, e-proteobactiria, Actinobacteria, and Flavobacteria.

关 键 词：胜利油田 石油降解菌 降解率 多样性 优势菌群 

分 类 号：X172[环境科学与工程—环境科学]