印度洋深海热液区可培养细菌的分子鉴定与系统发育分析  

Molecular Identification and Phylogenetic Analysis of Cultivable Bacteria From India Ocean Hydrothermal Vents

作　　者：刘艳[1,2,3] 黄晓航[2,3] 何培青[2,3] 彭亚林[2,3] 林学政[2,3] 安利国[1] 

机构地区：[1]山东师范大学生命科学学院动物抗性生物学重点实验室,山东济南250014 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]海洋生物活性物质国家海洋局重点实验室,山东青岛266061

出　　处：《海洋科学进展》2009年第2期193-200,共8页Advances in Marine Science

基　　金：中国大洋协会洋中脊综合调查与研究项目--深海热液区微生物的营养和生存策略研究(DYXM-115-02);国家海洋局青年海洋科学基金资助项目--深海热液区利用还原性乙酰辅酶A碳同化途径的化能自养细菌及其关键酶基因特征的研究(2008111)

摘　　要：采用MMJ HS寡营养培养基从印度洋深海热液区沉积物和热液硫化物中分离获得16株细菌,通过16SrDNA序列比对和生理生化分析,对它们进行了鉴定并构建了系统发育树。结果表明,12株细菌属于γ-变型菌(γ-Gammaproteobacteria),其中6株属于盐单胞菌属(Halomonas),4株属于嗜冷杆菌属(Psychrobacter),2株属于食碱菌属(Alcanivorax);其余4株属于芽孢杆菌(Bacillus),其中1株为地衣芽孢杆菌(Bacillus licheniformis)。分离获得的16株细菌中,4株为革兰氏阳性细菌,12株为革兰氏阴性细菌;H2S反应、吲哚测定反应、M.R和V-P均呈阴性。本研究为深入认识和开发利用深海热液区微生物资源奠定了基础。Totally 16 bacteria strains are isolated from sediments and sulfide of India Ocean hydrothermal vent by using MJHS oligo-medium. The identification of these bacteria is carried out, and their phyloge- netic tree is determined based on the comparison of 16S rDNA gene sequences and the physiological and bi- ochemical analysis. The results show that 12 bacteria strains belong to 7 proteobacteria,of which 6 are of genus Halomonas, 4 belong to genus Psychrobacte, 2 belong to genus Alcanivorax. The rest 4 strains are of Bacillus,of which 1 is Bacillus licheniformis. Among these 16 strains,4 strains are gram-negative and the rest are gram positive. It is learnt from the indole test,H2S test,MR test and VP test that all of them are negative. Therefore a foundation has been laid for further study and development of microbial resources in the deep-sea hydrothermal vents.

关 键 词：深海热液区 可培养 16S RDNA基因 细菌多样性 

分 类 号：Q934.2[生物学—微生物学]